PDK4 Antibody - BSA Free

PDK4 (pyruvate dehydrogenase kinase isoform 4) is expressed ubiquitously in the mitochondrion matrix of tissues with highest expression seen in liver, heart and skeletal muscles. PDK4 is regulated by glucocorticoids, retinoic acid and insulin, and is found elevated with diabetes, fasting and other conditions where fatty acids act as energy source. Under starvation/diabetic conditions, enhanced glucocorticoids levels activate glucocorticoid receptor thereby recruiting p300/CBP coactivator complex to PDK4 gene where it cooperatively interact with FOXO factors and activated RARs to make a stable transcriptional initiation complex responsible for PDK4 activation. Insulin promotes inactivation and translocation of FOXO factors from nucleus to cytoplasm and thereby suppresses glucocorticoid-stimulated PDK4 gene transcription. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex (PDC) via its E1 alpha subunit's phosphorylation, thus contributing to glucose metabolism regulation. Insulin resistance is associated with PDC dysregulation in skeletal muscles and excessive insulin on the other hand downregulates PDK4 expression. PDK4 exerts conservation of glucose as well as three-carbon compounds such as lactate, alanine, pyruvate etc. which serve as substrates for gluconeogenesis and this conservation helps maintain euglycemia during starvation, however, exacerbates hyperglycemia in diabetes.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 10 of 14. Show All 14 Publications. Collapse Publications.

Publications using NBP1-07047	Applications	Species
Toshihide Kashihara, Risa Mukai, Shin-ichi Oka, Peiyong Zhai, Yasuki Nakada, Zhi Yang, Wataru Mizushima, Tsutomu Nakahara, Junco S. Warren, Maha Abdellatif, Junichi Sadoshima YAP mediates compensatory cardiac hypertrophy through aerobic glycolysis in response to pressure overload The Journal of Clinical Investigation 2022-03-15 [PMID: 35133975]
Zhu L, An J, Chinnarasu S et al. Expressing the Human Cholesteryl Ester Transfer Protein Minigene Improves Diet-Induced Fatty Liver and Insulin Resistance in Female Mice Frontiers in physiology 2022-01-10 [PMID: 35082691] (WB, Mouse)	WB	Mouse
GOnczi M, Csemer A, SzabO L et al. Astaxanthin Exerts Anabolic Effects via Pleiotropic Modulation of the Excitable Tissue International journal of molecular sciences 2022-01-14 [PMID: 35055102] (WB, Mouse)	WB	Mouse
Strowitzki MJ, Radhakrishnan P, Pavicevic S et al. High hepatic expression of PDK4 improves survival upon multimodal treatment of colorectal liver metastases Br. J. Cancer 2019-02-27 [PMID: 30808993] (WB, Human)	WB	Human
Svensson K, Dent JR, Tahvilian S et al. Defining the contribution of skeletal muscle pyruvate dehydrogenase alpha 1 (Pdha1) to exercise performance and insulin action Am. J. Physiol. Endocrinol. Metab. 2018-08-28 [PMID: 30153068] (WB, Mouse)	WB	Mouse
Trinidad AG, Whalley N, Rowlinson R et al. Pyruvate dehydrogenase kinase 4 exhibits a novel role in the activation of mutant KRAS, regulating cell growth in lung and colorectal tumour cells Oncogene 2017-11-02 [PMID: 28692044] (WB, Mouse)	WB	Mouse
Niku, M, Pajari, A M Et al. Western diet enhances intestinal tumorigenesis in Min/+ mice, associating with mucosal metabolic and inflammatory stress and loss of Apc heterozygosity. J Nutr Biochem 2017-01-01 [PMID: 27833053] (ICC/IF, Mouse) Details: Citation using the HRP format of this antibody.	ICC/IF	Mouse
Golias T, Papandreou I, Sun R et al. Hypoxic repression of pyruvate dehydrogenase activity is necessary for metabolic reprogramming and growth of model tumours. Sci Rep. 2016-08-08 [PMID: 27498883] (WB, Human)	WB	Human
Oikawa S, Mano A, Takahashi R, Kakinuma Y. Remote ischemic preconditioning with a specialized protocol activates the non-neuronal cardiac cholinergic system and increases ATP content in the heart Int. Immunopharmacol. 2015-06-11 [PMID: 26072685] (WB, Mouse)	WB	Mouse
Hou X, Zhang L, Han L et al. Differential function of pyruvate dehydrogenase kinases during mouse oocyte maturation. J. Cell. Sci. 2015-05-19 [PMID: 25991547] (ICC/IF, Mouse)	ICC/IF	Mouse
Ho N, Coomber BL. Pyruvate dehydrogenase kinase expression and metabolic changes following dichloroacetate exposure in anoxic human colorectal cancer cells. Exp Cell Res 2015-02-01 [PMID: 25536473] (Human)		Human
Kainulainen H, Papaioannou Kg, Silvennoinen M et al. Myostatin/activin blocking combined with exercise reconditions skeletal muscle expression profile of mdx mice. Mol. Cell. Endocrinol. 2014-10-07 [PMID: 25304272] (WB, Mouse)	WB	Mouse
Rinnankoski-Tuikka R, Silvennoinen M, Torvinen S et al. Effects of high-fat diet and physical activity on pyruvate dehydrogenase kinase-4 in mouse skeletal muscle Nutr Metab (Lond) 2012-06-09 [PMID: 22682013] (WB, Mouse)	WB	Mouse
Connaughton S, Chowdhury F, Attia R et al. Regulation of pyruvate dehydrogenase kinase isoform 4 (PDK4) gene expression by glucocorticoids and insulin. Mol Cell Endocrinol. 2010-01-01 [PMID: 19703515]
Show All 14 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Thao Luu	WB	Mouse	04/05/2018	View Summary Application Western Blot Sample Tested Liver Species Mouse Lot B-4 Comments Comments We used the NuPAGE gel 4-12% for our Westerns.
	3 reviewed by: Verified Customer	WB	Human	08/22/2015	View Summary Application Western Blot Sample Tested Human cancer cell whole cell lysate Species Human

Array NBP1-07047

• PDK4 Antibodies
• PDK4 ELISA Kits
• PDK4 Lysate
• PDK4 Proteins