PDK4 Antibodies
We offer PDK4 Antibodies for use in common research applications: ELISA, ICC/IF (Negative), Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each PDK4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PDK4 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the PDK4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PDK4 polyclonal antibodies and browse our PDK4 monoclonal antibody catalog.
Alternate Names for PDK4 Antibodies
anti-PDK4 antibody, anti-[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.2 antibody, anti-FLJ40832 antibody, anti-Pyruvate dehydrogenase kinase isoform 4 antibody, anti-pyruvate dehydrogenase kinase, isoenzyme 4 antibody, anti-pyruvate dehydrogenase kinase, isozyme 4 antibody, anti-pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial antibody
6 Results for "pdk4" in Primary Antibodies
![Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0002.jpg "Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.")
![Western Blot: PDK4 Antibody [NBP1-07047] - Mouse liver. NuPAGE 4-12%. This image was submitted via customer review.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0003.jpg)
![Western Blot: PDK4 Antibody [NBP1-07047] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. * = vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0004.jpg)
![Western Blot: PDK4 Antibody - BSA Free [NBP1-07047] - Genetic manipulations can alter PDH E1 alpha phosphorylation in response to hypoxia.(a) MIA PaCa-2 control (Hg), silenced HIF1 alpha (shHIF1 alpha ) or silenced PDHK1 (shPDHK1) & PDP1 overexpressing cells were incubated for 16 h in normoxia or hypoxia (0.5% O2) & phospho-serine E1 alpha detected by Western blot showing that all three modifications can have an inhibitory effect on hypoxic Ser232 phosphorylation. (b) Increased pSer232-E1 alpha detection after E1 alpha immunoprecipitation from lysates of cells treated as in (a). (c) The same analysis as in (a) after treatment with 16 h 1 mM DMOG. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27498883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07047_rabbit-polyclonal-pdk4-antibody-31020241534333.jpg)
![Western Blot: PDK4 Antibody - BSA Free [NBP1-07047] - Hypoxia inhibits mitochondrial OCR & PDH activity & induces PDHK1 protein & activity.(a) Ratio of oxygen consumption rate (OCR) to extracellular acidification rate (ECAR) measured by Seahorse XF in MIA PaCa-2, PANC-1, & SU.86.86 cell lines in high (25 mM) or low (0.5 mM) glucose incubated overnight with or without 1 mM DMOG. (mean ± SEM, two-tailed Student’s t-test, **p < 0.01, ***p < 0.001) (b) Cell-based PDH activity assay in cells incubated 16 h in normoxia, hypoxia (0.5% O2) or 1 mM DMOG. (mean ± SD, one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001) (c) Western blots of HIF alpha isoforms, pyruvate dehydrogenase kinase isoforms (PDHKs), phosphatase (PDP1 – lower band *), target phosphorylated serine residues on E1 alpha & total E1 alpha after overnight incubation in normoxia or hypoxia (0.5% O2) at 25 or 5 mM glucose as indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27498883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07047_rabbit-polyclonal-pdk4-antibody-310202416171443.jpg)
![Western Blot: PDK4 Antibody [NBP1-07049] - Western Blot on human heart protein lysate.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07049-img0003.jpg "Western Blot: PDK4 Antibody [NBP1-07049] - Western Blot on human heart protein lysate.")
![Immunocytochemistry/Immunofluorescence: PDK4 Antibody [NBP1-07049] - PDK4 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-07049-img0004.jpg)
![Western Blot: PDK4 Antibody [NBP1-54723] - Titration: 0.2-1 ug/ml Positive Control: Transfected 293T.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-54723-img0005.jpg "Western Blot: PDK4 Antibody [NBP1-54723] - Titration: 0.2-1 ug/ml Positive Control: Transfected 293T.")
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP1-54723] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP1-54723-img0006.jpg)
![Western Blot: PDK4 Antibody [NBP1-54723] - Sample Type: Huh7 HepG2 (50ug) Primary Antibody Dilution: 1:500 Image Submitted By: Partha Kasturi University of Kansas Medical Center.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-54723-img0004.jpg)
![Western Blot: PDK4 Antibody [NBP2-93829] - Analysis of various lysates, using PDK4 antibody at 1:2350 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP2-93829-img0007.jpg "Western Blot: PDK4 Antibody [NBP2-93829] - Analysis of various lysates, using PDK4 antibody at 1:2350 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: PDK4 Antibody [NBP2-93829] - Immunofluorescence analysis of U-2 OS cells using PDK4 antibody (NBP2-93829) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93829-img0003.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Mouse heart using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0006.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Rat heart using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0004.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Human placenta using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0005.jpg)
![Western Blot: PDK4 Antibody [NBP2-95194] - Western blot analysis of extracts of various cell lines, using PDK4 antibody (NBP2-95194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PDK4 Antibody-Western Blot-NBP2-95194-img0003.jpg "Western Blot: PDK4 Antibody [NBP2-95194] - Western blot analysis of extracts of various cell lines, using PDK4 antibody (NBP2-95194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-95194] - Immunohistochemistry of paraffin-embedded human placenta using PDK4 Rabbit pAb (NBP2-95194) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-95194-img0002.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-95194] - Immunohistochemistry of paraffin-embedded rat heart using PDK4 Rabbit pAb (NBP2-95194) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-95194-img0001.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-49235] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-49235-img0002.jpg "Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-49235] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.")