![Western Blot: PDPK1 Antibody [NBP2-44132] - Detection of myc-tagged human PDK-1 at 60kDa in 10 Ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 C with the](http://images.novusbio.com/fullsize/PDPK1-Antibody-Western-Blot-NBP2-44132-img0001.jpg "Western Blot: PDPK1 Antibody [NBP2-44132] - Detection of myc-tagged human PDK-1 at 60kDa in 10 Ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 C with the")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This affinity purified antibody is directed against human PDK-1. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This affinity purified PDK-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human PDK-1 protein. (Uniprot: Q15118) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PDPK1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~60 kDa in size corresponding to human PDK-1 protein by western blotting in the appropriate cell lysate or extract. Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation. Reactivity in other immunoassays is unknown. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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