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PDE6A Antibody

Images

 
Independent Antibodies: Immunohistochemistry-Paraffin: PDE6A Antibody [NBP1-87312] - Staining of human cerebral cortex, endometrium, eye, retina and liver using Anti-PDE6A antibody NBP1-87312 (A) shows similar ...read more
Western Blot: PDE6A Antibody [NBP1-87312] - Analysis in control (vector only transfected HEK293T lysate) and PDE6A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: PDE6A Antibody [NBP1-87312] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: PDE6A Antibody [NBP1-87312] - Staining of human retina shows moderate cytoplasmic positivity in rods.
Immunohistochemistry-Paraffin: PDE6A Antibody [NBP1-87312] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: PDE6A Antibody [NBP1-87312] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, Pm, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

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PDE6A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TLMESLTQFLGWSVLNPDTYESMNKLENRKDIFQDIVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEILQAELPDADKYEINKFHFSDLPLTELELVKCGIQMYYELKVVDKFHIPQEALVRFMYSLSKGYRKITYHN
Predicted Species
Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDE6A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID: 29212391)
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
PDE6A Protein (NBP1-87312PEP)
Publications
Read Publications using
NBP1-87312 in the following applications:

Reactivity Notes

Use in Monkey reported in scientific literature (PMID:35024589). Mouse reactivity reported in scientific literature (PMID: 26188004).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PDE6A Antibody

  • CGPR-A
  • EC 3.1.4
  • EC 3.1.4.35
  • GMP-PDE alpha
  • PDE V-B1
  • PDE6A
  • PDEA
  • PDEAcGMP phosphodiesterase alpha subunit
  • phosphodiesterase 6A, cGMP-specific, rod, alpha
  • rod cGMP-specific 3'-5'-cyclic phosphodiesterase subunit alpha
  • rod photoreceptor cGMP phosphodiesterase alpha subunit
  • RP43

Background

Phosphodiesterase 6A, a catalytic subunit of the photoreceptor phosphodiesterase, has been shown to affect functioning of eye retina as the alpha subunit of the heterotetrameric rod photoreceptor. Mutations in PDE6A have been identified as a cause of autosomal recessive retinitis pigmentosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-87312
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Applications: WB, IHC

Publications for PDE6A Antibody (NBP1-87312)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PDE6A Antibody (NBP1-87312) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDE6A