![Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of skeletal muscle lysates and His-tagged recombinant proteins using mouse parvalbumin mAb, dilution 1:1,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] full length human parvalbumin, [4] full length human calretinin, and [5] full length human calbindin. A band at 12kDa is detected in in muscle lysate and one at 18kDa in the His-tagged recombinant parvalbumin protein lane as expected since the His-tag and other vector derived sequence adds about 6kDa to the molecule. Note that the parvalbumin antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-3C9-Western-Blot-NBP2-50038-img0005.jpg "Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of skeletal muscle lysates and His-tagged recombinant proteins using mouse parvalbumin mAb, dilution 1:1,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] full length human parvalbumin, [4] full length human calretinin, and [5] full length human calbindin. A band at 12kDa is detected in in muscle lysate and one at 18kDa in the His-tagged recombinant parvalbumin protein lane as expected since the His-tag and other vector derived sequence adds about 6kDa to the molecule. Note that the parvalbumin antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.")
| Reactivity | Hu, Mu, Rt, Po, Bv, EqSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 3C9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full-length recombinant human Parvalbumin expressed in and purified from E. coli. [UniProt# P20472] |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PVALB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In WB, this antibody recognizes a band at 12 kDa in skeletal muscle lysates, which represents Parvalbumin. Note, this antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences. |
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| Theoretical MW | 12 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publications using NBP2-50038 | Applications | Species |
|---|---|---|
| Sun XY, Liu L, Song YT et al. Two parallel medial prefrontal cortex-amygdala pathways mediate memory deficits via glutamatergic projection in surgery mice Cell reports 2023-06-30 [PMID: 37392387] (Mouse) | Mouse | |
| Sun X, Liu L, Wu T et al. Two Parallel Medial Prefrontal Cortex-Amygdala Circuits Mediate Memory Deficit via Glutamatergic Projection in Surgery Mice SSRN Electronic Journal 2022-10-12 (IF/IHC, Mouse) | IF/IHC | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for Parvalbumin Antibody (NBP2-50038)Find related products by research area.
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Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of rat cerebellum section stained with mouse mAb to parvalbumin, NBP2-50038, dilution 1:1,000, in green, and costained with chicken pAb to calbindin, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone and so appear are green.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-3C9-Immunocytochemistry-Immunofluorescence-NBP2-50038-img0004.jpg "Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of rat cerebellum section stained with mouse mAb to parvalbumin, NBP2-50038, dilution 1:1,000, in green, and costained with chicken pAb to calbindin, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone and so appear are green.")
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Adult rat cerebellum floating section was stained with NBP2-50038 at 1:1,000 (green). Parvalbumin is prominently expressed in the dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-3C9-Immunocytochemistry-Immunofluorescence-NBP2-50038-img0003.jpg "Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Adult rat cerebellum floating section was stained with NBP2-50038 at 1:1,000 (green). Parvalbumin is prominently expressed in the dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue is a DNA stain.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")