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![Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NB120-11427-img0002.jpg "Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.")
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in U251 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0008.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin and parvalbumin antibodies shown in layer 2/3 at 63x magnification (scale = 10 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2013.00156/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in C6 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in Hela Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0007.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human cerebellum tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0003.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human skeletal muscle tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0004.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human tonsil tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin & parvalbumin antibodies shown in layer 2/3 at 63× magnification (scale = 10 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24065886), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11427_rabbit-polyclonal-parvalbumin-antibody-310202415284515.jpg)
![Immunohistochemistry: Parvalbumin Antibody - BSA Free [NB120-11427] - Female Ambra1+/− mice show reduced numbers of hippocampal PV interneurons. a Nissl staining of dorsal hippocampus coronal sections from WT & Ambra1+/− female mice (scale bar: 500 μm). b Subdivision of hippocampal layers (stratum oriens, CA1 pyramidal layer, stratum radiatum, stratum lacunosum moleculare, stratum moleculare, dentate gyrus upper blade, hilus, dentate gyrus lower blade), corresponding to the area depicted in a (scale bar: 100 μm). Ambra1+/− females show normal hippocampal layering (n = 3 mice per genotype). c Double labeling for Ambra1 (green) & PV (red) in the CA1 from a WT female; sections were DAPI-counterstained (blue) for neuronal nuclei (scale bar: 50 μm). The higher magnification image (scale bar, 5 μm) from the stratum pyramidale shows a PV neuron (right; merge in orange) & an unidentified neuron (left), both expressing Ambra1. The DAPI signal is omitted for clarity & neuron borders are indicated by a dash line (n = 3 mice; three sections per animal). d, e Immunohistochemical labeling of PV in a coronal brain section of dorsal hippocampus obtained from female (d) & male (e) mice (scale bars: 500 μm). On the right are higher magnification images (scale bars: 50 μm). The plots show stereological quantification of PV interneurons (n = 6 mice per genotype & per gender; 14 sections per animal). The reduction in PV interneuron numbers is selective for female Ambra1+/− (two-tailed unpaired t test: *P = 0.043) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29488136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11427_rabbit-polyclonal-parvalbumin-antibody-310202416205149.jpg)
![Western Blot: Parvalbumin Antibody [NBP2-50036] - Analysis of skeletal muscle lysates and His-tagged recombinant human proteins using chicken parvalbumin pAb, dilution 1:2,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] recombinant full length human parvalbumin, [4] recombinant full length human calretinin and [5] recombinant full length human calbindin. Band at 12kDa in muscle lysate is native parvalbumin and 18kDa band in the next lane is His-tagged recombinant parvalbumin. The parvalbumin antibody binds parvalbumin strongly but is not cross-reactive with the related calcium binding proteins calretinin and calbindin.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NBP2-50036-img0005.jpg "Western Blot: Parvalbumin Antibody [NBP2-50036] - Analysis of skeletal muscle lysates and His-tagged recombinant human proteins using chicken parvalbumin pAb, dilution 1:2,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] recombinant full length human parvalbumin, [4] recombinant full length human calretinin and [5] recombinant full length human calbindin. Band at 12kDa in muscle lysate is native parvalbumin and 18kDa band in the next lane is His-tagged recombinant parvalbumin. The parvalbumin antibody binds parvalbumin strongly but is not cross-reactive with the related calcium binding proteins calretinin and calbindin.")
![Immunohistochemistry Free-Floating: Parvalbumin Antibody [NBP2-50036] - Analysis of a rat cerebellum section stained with chicken pAb to parvalbumin, NBP2-50036, dilution 1:2,000, in green, and costained with mouse mAb to GFAP, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. NBP2-50036 antibody labels the perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astrocytes.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunofluorescence-NBP2-50036-img0004.jpg)
![Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of skeletal muscle lysates and His-tagged recombinant proteins using mouse parvalbumin mAb, dilution 1:1,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] full length human parvalbumin, [4] full length human calretinin, and [5] full length human calbindin. A band at 12kDa is detected in in muscle lysate and one at 18kDa in the His-tagged recombinant parvalbumin protein lane as expected since the His-tag and other vector derived sequence adds about 6kDa to the molecule. Note that the parvalbumin antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-3C9-Western-Blot-NBP2-50038-img0005.jpg "Western Blot: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of skeletal muscle lysates and His-tagged recombinant proteins using mouse parvalbumin mAb, dilution 1:1,000 (Green): [1] protein standard (Red), [2] mouse muscle, [3] full length human parvalbumin, [4] full length human calretinin, and [5] full length human calbindin. A band at 12kDa is detected in in muscle lysate and one at 18kDa in the His-tagged recombinant parvalbumin protein lane as expected since the His-tag and other vector derived sequence adds about 6kDa to the molecule. Note that the parvalbumin antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.")
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Analysis of rat cerebellum section stained with mouse mAb to parvalbumin, NBP2-50038, dilution 1:1,000, in green, and costained with chicken pAb to calbindin, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone and so appear are green.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-3C9-Immunocytochemistry-Immunofluorescence-NBP2-50038-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody (3C9) [NBP2-50038] - Adult rat cerebellum floating section was stained with NBP2-50038 at 1:1,000 (green). Parvalbumin is prominently expressed in the dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-3C9-Immunocytochemistry-Immunofluorescence-NBP2-50038-img0003.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Analysis in human parathyroid gland and liver tissues. Corresponding Parvalbumin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Analysis in human parathyroid gland and liver tissues. Corresponding Parvalbumin RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Parvalbumin Antibody [NBP2-33529] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NBP2-33529-img0015.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0014.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0008.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human cerebellum shows strong positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0011.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human cerebral cortex shows strong positivity in a small subset of neurons.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0012.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NBP2-33529] - Staining of human kidney shows strong positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NBP2-33529-img0013.jpg)
![Western Blot: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Analysis of extracts of various cell lines, using Parvalbumin (PVALB) antibody (NBP3-15664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Western-Blot-NBP3-15664-img0004.jpg "Western Blot: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Analysis of extracts of various cell lines, using Parvalbumin (PVALB) antibody (NBP3-15664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Mouse brain using Parvalbumin (PVALB) Rabbit mAb (NBP3-15664) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Immunohistochemistry-Paraffin-NBP3-15664-img0003.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Rat brain using Parvalbumin (PVALB) Rabbit mAb (NBP3-15664) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Immunohistochemistry-Paraffin-NBP3-15664-img0001.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody (6C7K6) [NBP3-15664] - Human appendix using Parvalbumin (PVALB) Rabbit mAb (NBP3-15664) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-6C7K6-Immunohistochemistry-Paraffin-NBP3-15664-img0002.jpg)
![Western Blot: Parvalbumin Antibody [NB100-1541] - (0.01ug/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NB100-1541-img0002.jpg "Western Blot: Parvalbumin Antibody [NB100-1541] - (0.01ug/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Parvalbumin Antibody [NB100-1541] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Flow-Cytometry-NB100-1541-img0001.jpg)
![Western Blot: Parvalbumin Antibody (C12) [NBP2-79824] - Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-C12-Western-Blot-NBP2-79824-img0001.jpg "Western Blot: Parvalbumin Antibody (C12) [NBP2-79824] - Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: Parvalbumin Antibody (C12) [NBP2-79824] - Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-C12-Immunohistochemistry-NBP2-79824-img0002.jpg)
![Immunohistochemistry: Parvalbumin Antibody (C12) [NBP2-79824] - Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (NBP2-79824). Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (NBP2-79824) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-C12-Immunohistochemistry-NBP2-79824-img0003.jpg)
![Western Blot: Parvalbumin Overexpression Lysate (Adult Normal) [NBP2-10851] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Parvalbumin.](http://images.novusbio.com/fullsize/Parvalbumin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10851-img0001.jpg "Western Blot: Parvalbumin Overexpression Lysate (Adult Normal) [NBP2-10851] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Parvalbumin.")
![SDS-Page: Recombinant Human Parvalbumin Protein [NBP1-50865] - 15% SDS-Page (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-Parvalbumin-Protein-SDS-Page-NBP1-50865-img0001.jpg "SDS-Page: Recombinant Human Parvalbumin Protein [NBP1-50865] - 15% SDS-Page (3ug)")
![ELISA: Human Parvalbumin ELISA Kit (Colorimetric) [NBP3-18221] - Standard Curve Reference](http://images.novusbio.com/fullsize/Human-Parvalbumin-ELISA-Kit-Colorimetric-ELISA-NBP3-18221-img0001.jpg "ELISA: Human Parvalbumin ELISA Kit (Colorimetric) [NBP3-18221] - Standard Curve Reference")