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Pancreatic Amylase Alpha Antibody (OTI1E3)

Images

 
Western Blot: Pancreatic Amylase Alpha Antibody (1E3) [NBP2-46518] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMY2A .
Immunohistochemistry: Pancreatic Amylase Alpha Antibody (1E3) [NBP2-46518] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI1E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Pancreatic Amylase Alpha Antibody (OTI1E3) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 135-408 of human AMY2A (NP_000690) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
AMY2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
55.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4
using
NBP2-46518 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pancreatic Amylase Alpha Antibody (OTI1E3)

  • AMY2
  • AMY2A amylase, alpha 2A (pancreatic)
  • AMY2B
  • PA
  • pancreatic alpha-amylase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pancreatic Amylase Alpha Antibody (NBP2-46518) (0)

There are no publications for Pancreatic Amylase Alpha Antibody (NBP2-46518).
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Reviews for Pancreatic Amylase Alpha Antibody (NBP2-46518) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Human and Rat.

Reviews using NBP2-46518:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Mouse
(1)
Human and Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Pancreatic Amylase Alpha NBP2-46518
Enlarge
3
reviewed by:
Scott Messenger
ICC Mouse 06/09/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse pancreas in OCT
SpeciesMouse

Comments

Commentsmouse pancreas, 11 um section, 1/200 primary, 1/200 secondardy 568. DAPI labeled nuclei. Unsure if zymogen granules are marked. Epi-Fluorescence 60 x objective
Western Blot Pancreatic Amylase Alpha NBP2-46518
Enlarge
5
reviewed by:
Scott Messenger
WB Human and Rat 05/05/2017
View

Summary

ApplicationWestern Blot
Sample Testedacini (group of isolated acinar cells),AR42J rat acinar pancreatic cancer cell line
SpeciesHuman and Rat

Comments

Commentshuman or rat acini, AR42J left untreated or 72 h with dexamethasone. 50 ug whole cell lysate. primary 1/1000

Product General Protocols

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Video Protocols

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FAQs for Pancreatic Amylase Alpha Antibody (NBP2-46518) (0)

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Scott Messenger
06/09/2017
Application: ICC
Species: Mouse

Scott Messenger
05/05/2017
Application: WB
Species: Human and Rat

Bioinformatics

Gene Symbol AMY2A