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Pallidin Antibody (15G2)

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Western Blot: Pallidin Antibody (15G2) [NB120-13261] - Analysis of Syntaxin 13 (15G2), mAb : Lane 1: MW Marker, Lane 2: Rat Brain Lysate Lane 3: Mouse Brain Lysate, Lane 4: CHO-K1 Cell Lysate (Heat Shocked)
Immunohistochemistry-Paraffin: Pallidin Antibody (15G2) [NB120-13261] - Analysis of human placenta tissue stained with Syntaxin 13, mAb (15G2) at 20 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, HaSpecies Glossary
Applications WB, IHC
Clone
15G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Pallidin Antibody (15G2) Summary

Immunogen
Recombinant full length protein (Rat).
Localization
Tubular early and recycling endosomes and endosomal vacuoles.
Specificity
Syntaxin 13 (15G2)
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
BLOC1S6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100-1:2000
Application Notes
WB: Detects a band of approximately 38 kDa. Additional bands of 27 and 37 kDa may also be recognized, and are believed to correspond to degradation products of syntaxin 13.
Control
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Cross-reacts with Mouse, Rat and Dog. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Pallidin Antibody (15G2)

  • pallid (mouse) homolog, pallidin
  • Pallid protein homolog
  • PALLID
  • pallidin homolog (mouse)
  • pallidin
  • PAsyntaxin 13 binding protein 1
  • syntaxin 13-interacting protein pallid
  • Syntaxin 13-interacting protein

Background

Syntaxin 13 is an integral membrane protein that belongs to the t-SNARE family, a group of proteins involved in protein transport. Confocal immunofluoresence and electron microscopy studies have shown that syntaxin 13 is primarily localized to tubular early and recycling endosomes, where it colocalizes with transferrin receptor, and it is also localized in endosomal vacuoles. Syntaxin 13 has been found to be expressed in all tissues, with higher levels of the protein found in brain, lung, spleen, thymus and testes. Immunoprecipitation studies show that syntaxin 13 complexes with beta-SNAP, VAMP2/3, and SNAP25. The binding of this complex to alpha-SNAP and NSF is terminated in the presence of ATP. These results suggest that syntaxin 13 is a SNARE protein which mediates the recycling protein flow through tubulo vesicular recycling endosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BLOC1S6
Entrez