Pallidin Antibody (15G2) Summary
Immunogen |
Recombinant full length protein (Rat). |
Localization |
Tubular early and recycling endosomes and endosomal vacuoles. |
Specificity |
Syntaxin 13 (15G2) |
Isotype |
IgG |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
BLOC1S6 |
Purity |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry
- Immunohistochemistry-Paraffin
- Western Blot 1:100-1:2000
|
Application Notes |
WB: Detects a band of approximately 38 kDa. Additional bands of 27 and 37 kDa may also be recognized, and are believed to correspond to degradation products of syntaxin 13. |
Control |
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Reactivity Notes
Cross-reacts with Mouse, Rat and Dog. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS and 50% Glycerol |
Preservative |
0.09% Sodium Azide |
Concentration |
1.0 mg/ml |
Purity |
Protein A or G purified |
Alternate Names for Pallidin Antibody (15G2)
Background
Syntaxin 13 is an integral membrane protein that belongs to the t-SNARE family, a group of proteins involved in protein transport. Confocal immunofluoresence and electron microscopy studies have shown that syntaxin 13 is primarily localized to tubular early and recycling endosomes, where it colocalizes with transferrin receptor, and it is also localized in endosomal vacuoles. Syntaxin 13 has been found to be expressed in all tissues, with higher levels of the protein found in brain, lung, spleen, thymus and testes. Immunoprecipitation studies show that syntaxin 13 complexes with beta-SNAP, VAMP2/3, and SNAP25. The binding of this complex to alpha-SNAP and NSF is terminated in the presence of ATP. These results suggest that syntaxin 13 is a SNARE protein which mediates the recycling protein flow through tubulo vesicular recycling endosomes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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