![Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NB120-2940] - Characterization of PA28alpha and PSMD11 overexpressing (OE) mice. a Western blots of proteasomal subunits in retinal lysates containing 30ug total protein. Bands were visualized using the LiCor Odyssey imaging system. Each protein was analyzed in at least 3 pairs of 1-month-old WT and overexpressing animals. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-04117-8), licensed under a CC-BY license](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-Western-Blot-NB120-2940-img0008.jpg "Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NB120-2940] - Characterization of PA28alpha and PSMD11 overexpressing (OE) mice. a Western blots of proteasomal subunits in retinal lysates containing 30ug total protein. Bands were visualized using the LiCor Odyssey imaging system. Each protein was analyzed in at least 3 pairs of 1-month-old WT and overexpressing animals. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-04117-8), licensed under a CC-BY license")
| Reactivity | Hu, Mu, PmSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic Peptide: M(1) A K P C G V R L S G E A R(14) |
| Specificity | Detects recombinant human proteasome 11S REG beta. This does not detect endogenous levels of proteasome 11S REG beta nor does it detect other 11S REG subunits. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PSME2 |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Detects an approx. 28 kDa protein representing recombinant human proteasome 11S REG beta. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Ammonium sulfate precipitation |
| Publication using NB120-2940 | Applications | Species |
|---|---|---|
| Lobanova ES, Finkelstein S, Li J et al. Increased proteasomal activity supports photoreceptor survival in inherited retinal degeneration Nat Commun 2018-04-30 [PMID: 29712894] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for PA28 Activator beta Subunit/PSME2 Antibody (NB120-2940)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PA28 Activator beta Subunit/PSME2 Antibody [NB120-2940] - Analysis of Proteasome 11S REG beta (green) showing staining in the cytoplasm and nucleus of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Proteasome 11S REG beta polyclonal antibody in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-Immunocytochemistry-Immunofluorescence-NB120-2940-img0007.jpg "Immunocytochemistry/Immunofluorescence: PA28 Activator beta Subunit/PSME2 Antibody [NB120-2940] - Analysis of Proteasome 11S REG beta (green) showing staining in the cytoplasm and nucleus of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Proteasome 11S REG beta polyclonal antibody in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.")
![Immunocytochemistry/Immunofluorescence: PA28 Activator beta Subunit/PSME2 Antibody [NB120-2940] - Analysis of Proteasome 11S REG beta (green) showing staining in the cytoplasm and nucleus of Neuro-2a cells.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-Immunocytochemistry-Immunofluorescence-NB120-2940-img0004.jpg "Immunocytochemistry/Immunofluorescence: PA28 Activator beta Subunit/PSME2 Antibody [NB120-2940] - Analysis of Proteasome 11S REG beta (green) showing staining in the cytoplasm and nucleus of Neuro-2a cells.")
![Immunocytochemistry/Immunofluorescence: PA28 Activator beta Subunit/PSME2 Antibody [NB120-2940] - Analysis of Proteasome 11S REG beta (green) showing staining in the cytoplasm and nucleus of MCF-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Proteasome 11S REG beta polyclonal antibody in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/fullsize/PA28-Activator-beta-Subunit-PSME2-Antibody-Immunocytochemistry-Immunofluorescence-NB120-2940-img0005.jpg "Immunocytochemistry/Immunofluorescence: PA28 Activator beta Subunit/PSME2 Antibody [NB120-2940] - Analysis of Proteasome 11S REG beta (green) showing staining in the cytoplasm and nucleus of MCF-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Proteasome 11S REG beta polyclonal antibody in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")