Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: p97/VCP Antibody [NB100-1557] - Sections of human ovarian carcinoma (upper) and mouse squamous cell carcinoma (lower). Antibody: Affinity purified rabbit anti-VCP used at a dilution of ...read more
p97/VCP Antibody [NB100-1557] - Detection of human and mouse VCP by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. ...read more
p97/VCP Antibody [NB100-1557] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. ...read more
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Valosin-Containing Protein using the numbering given in entry NP_009057.1 (GeneID 7415).
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. p97/VCP antibody validated for WB from a verified customer review.
Theoretical MW
89 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rat reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified
Alternate Names for p97/VCP Antibody
15S Mg(2+)-ATPase p97 subunit
IBMPFD
IBMPFDMGC8560
MGC131997
p97
TER ATPase
TERA
transitional endoplasmic reticulum ATPase
valosin containing protein
Valosin-containing proteinMGC148092
VCP
yeast Cdc48p homolog
Background
Valosin-containing protein (VCP) is a multifunctional protein that is proposed to be involved in vesicle transport and fusion for protein trafficking, 26S proteasome function in ubiquitin-based degradation pathways, and the assembly of peroxisomes. Mutations in the human VCP gene have been associated with diseases such as inclusion body myopathy, Paget disease of the bone, and frontotemporal dementia (IBMPFD). VCP can inhibit apoptosis and may play a role in tumorigenesis. VCP is also known as, Transitional Endoplasmic Reticulum ATPase, TER ATPase, TERA, 15S Mg (2+)-ATPase p97subunit, IBMPFD, Inclusion Body Myopathy associated with Paget Disease of Bone and Frontotemporal Dementia, MGC8560; MGC131997; MGC148092.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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