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p27/Kip1 Antibody (KIP1/769) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone
KIP1/769
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47769AF350
Alexa Fluor 405
NBP2-47769AF405
Alexa Fluor 488
NBP2-47769AF488
Alexa Fluor 532
NBP2-47769AF532
Alexa Fluor 594
NBP2-47769AF594
Alexa Fluor 647
NBP2-47769AF647
Alexa Fluor 700
NBP2-47769AF700
Alexa Fluor 750
NBP2-47769AF750
Allophycocyanin
NBP2-47769APC
Biotin
NBP2-47769B
CoraFluor 1
NBP2-47769CL1
DyLight 350
NBP2-47769UV
DyLight 405
NBP2-47769V
DyLight 488
NBP2-47769G
DyLight 550
NBP2-47769R
DyLight 594
NBP2-47769DL594
DyLight 650
NBP2-47769C
DyLight 680
NBP2-47769FR
DyLight 755
NBP2-47769IR
FITC
NBP2-47769F
HRP
NBP2-47769H
Janelia Fluor 525
NBP2-47769JF525
Janelia Fluor 549
NBP2-47769JF549
Janelia Fluor 585
NBP2-47769JF585
Janelia Fluor 646
NBP2-47769JF646
Janelia Fluor 669
NBP2-47769JF669
PE
NBP2-47769PE
PerCP
NBP2-47769PCP
Unconjugated
NBP2-44495-0.1mg
mFluor Violet 450 SE
NBP2-47769MFV450
mFluor Violet 500 SE
NBP2-47769MFV500
mFluor Violet 610 SE
NBP2-47769MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44495-0.1mg
Azide and BSA Free
NBP2-47769-0.1mg
IHC-Prediluted
NBP2-48399
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p27/Kip1 Antibody (KIP1/769) [Janelia Fluor® 635] Summary

Immunogen
Recombinant full-length human p27/Kip1 protein (Uniprot: P46527)
Localization
Nuclear
Specificity
This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This monoclonal antibody is excellent for staining of formalin-fixed tissues.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1B
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for p27/Kip1 Antibody (KIP1/769) [Janelia Fluor® 635]

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Background

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDKN1B