![Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (KIP1/769) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47769-img0004.jpg "Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (KIP1/769) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | KIP1/769 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44495). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant full-length human p27/Kip1 protein (Uniprot: P46527) |
| Localization | Nuclear |
| Specificity | This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This monoclonal antibody is excellent for staining of formalin-fixed tissues. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CDKN1B |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for p27/Kip1 Antibody (NBP2-47769)Find related products by research area.
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| Gene Symbol | CDKN1B |
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Rat Colon stained with p27 Monoclonal Antibody (KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47769-img0008.jpg "Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Rat Colon stained with p27 Monoclonal Antibody (KIP1/769)")
![Flow (Intracellular): p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - An intracellular stain was performed on Jurkat cells with p27/Kip1 Antibody (KIP1/769) NBP2-47769PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. Image using the Azide and BSA Free form of this antibody.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Azide-and-BSA-Free-Flow-Intracellular-NBP2-47769-img0003.jpg "Flow (Intracellular): p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - An intracellular stain was performed on Jurkat cells with p27/Kip1 Antibody (KIP1/769) NBP2-47769PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. Image using the Azide and BSA Free form of this antibody.")
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Human Cervical cancer stained with p27 Monoclonal Antibody (KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47769-img0005.jpg "Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Human Cervical cancer stained with p27 Monoclonal Antibody (KIP1/769)")
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![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Human Prostate cancer stained with p27 Monoclonal Antibody (KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47769-img0007.jpg "Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Human Prostate cancer stained with p27 Monoclonal Antibody (KIP1/769)")
![Flow Cytometry: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Human p27 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (KIP1/769).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-KIP1-769-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47769-img0002.jpg "Flow Cytometry: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Human p27 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (KIP1/769).")
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