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p27/Kip1 Antibody (225501) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
225501
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2256U-100UG
Alexa Fluor 405
IC2256V-100UG
Alexa Fluor 488
IC2256G-100UG
Alexa Fluor 532
FAB2256X
Alexa Fluor 594
IC2256T-100UG
Alexa Fluor 647
IC2256R-100UG
Alexa Fluor 700
IC2256N-100UG
Alexa Fluor 750
IC2256S-100UG
Allophycocyanin
FAB2256A
Biotin
FAB2256B
CoraFluor 1
FAB2256CL1
DyLight 350
FAB2256D
DyLight 405
FAB2256E
DyLight 488
FAB2256K
DyLight 550
FAB2256L
DyLight 594
FAB2256M
DyLight 650
FAB2256W
DyLight 680
FAB2256Y
DyLight 755
FAB2256Z
FITC
FAB2256F
HRP
FAB2256H
Janelia Fluor 525
FAB2256JF525
Janelia Fluor 549
FAB2256I
Janelia Fluor 585
FAB2256JF585
Janelia Fluor 635
FAB2256JF635
Janelia Fluor 669
FAB2256JF669
PE
FAB2256P
PerCP
FAB2256C
Unconjugated
MAB2256
mFluor Violet 450 SE
FAB2256MFV450
mFluor Violet 500 SE
FAB2256MFV500
mFluor Violet 610 SE
FAB2256MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2256
Carrier Free
MAB2256
Carrier Free
MAB2256-SP
Carrier Free
IC2256N-100UG
Carrier Free
IC2256G-100UG
Carrier Free
IC2256V-100UG
Carrier Free
IC2256U-100UG
Carrier Free
IC2256T-100UG
Carrier Free
IC2256S-100UG
Carrier Free
IC2256R-100UG
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p27/Kip1 Antibody (225501) [Janelia Fluor® 646] Summary

Immunogen
E. coli-derived recombinant human p27
Met1-Thr198
Accession # P46527
Specificity
Detects human, mouse, and rat p27 in Western blots.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1B
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for p27/Kip1 Antibody (225501) [Janelia Fluor® 646]

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Background

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDKN1B