Home » p27/Kip1 » p27/Kip1 Antibodies » p27/Kip1 Antibody (225501) [Biotin]
Novus Biologicals products are now on bio-techne.com

p27/Kip1 Antibody (225501) [Biotin]

 Submit a Review

Images

 
There are currently no images for p27/Kip1 Antibody (FAB2256B).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
225501
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2256U-100UG
Alexa Fluor 405
IC2256V-100UG
Alexa Fluor 488
IC2256G-100UG
Alexa Fluor 532
FAB2256X
Alexa Fluor 594
IC2256T-100UG
Alexa Fluor 647
IC2256R-100UG
Alexa Fluor 700
IC2256N-100UG
Alexa Fluor 750
IC2256S-100UG
Allophycocyanin
FAB2256A
CoraFluor 1
FAB2256CL1
DyLight 350
FAB2256D
DyLight 405
FAB2256E
DyLight 488
FAB2256K
DyLight 550
FAB2256L
DyLight 594
FAB2256M
DyLight 650
FAB2256W
DyLight 680
FAB2256Y
DyLight 755
FAB2256Z
FITC
FAB2256F
HRP
FAB2256H
Janelia Fluor 525
FAB2256JF525
Janelia Fluor 549
FAB2256I
Janelia Fluor 585
FAB2256JF585
Janelia Fluor 635
FAB2256JF635
Janelia Fluor 646
FAB2256J
Janelia Fluor 669
FAB2256JF669
PE
FAB2256P
PerCP
FAB2256C
Unconjugated
MAB2256
mFluor Violet 450 SE
FAB2256MFV450
mFluor Violet 500 SE
FAB2256MFV500
mFluor Violet 610 SE
FAB2256MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2256
Carrier Free
MAB2256
Carrier Free
MAB2256-SP
Carrier Free
IC2256N-100UG
Carrier Free
IC2256G-100UG
Carrier Free
IC2256V-100UG
Carrier Free
IC2256U-100UG
Carrier Free
IC2256T-100UG
Carrier Free
IC2256S-100UG
Carrier Free
IC2256R-100UG
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

p27/Kip1 Antibody (225501) [Biotin] Summary

Immunogen
E. coli-derived recombinant human p27
Met1-Thr198
Accession # P46527
Specificity
Detects human, mouse, and rat p27 in Western blots.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1B
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Alternate Names for p27/Kip1 Antibody (225501) [Biotin]

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Background

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin p21/CIP1/CDKN1A Antibody (WA-1)
(24 Publications)
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TMED7 Antibody
NBP1-90949
Immunohistochemistry TMED7 Antibody
Immunohistochemistry TMED7 Antibody
Immunohistochemistry TMED7 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
Dynactin 6 Antibody
NBP1-80616
Immunohistochemistry-Paraffin Dynactin 6 Antibody
Western Blot Dynactin 6 Antibody
Western Blot Dynactin 6 Antibody
(2 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
SSSCA1 Antibody
(OTI2F5)
NBP2-03198
Flow Cytometry SSSCA1 Antibody (OTI2F5)
Flow Cytometry SSSCA1 Antibody (OTI2F5)
Flow Cytometry SSSCA1 Antibody (OTI2F5)
(1 Publication)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IFI27 Antibody
(CL14627)
NBP3-26876
Immunohistochemistry-Paraffin IFI27 Antibody (CL14627)
Immunohistochemistry-Paraffin IFI27 Antibody (CL14627)
Immunohistochemistry-Paraffin IFI27 Antibody (CL14627)
Species: Hu
Applications: IHC
26S proteasome
subunit 9 Antibody
NBP2-47560
Immunohistochemistry 26S proteasome subunit 9 Antibody
Immunohistochemistry-Paraffin 26S proteasome subunit 9 Antibody
Immunohistochemistry-Paraffin 26S proteasome subunit 9 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CDK2 Antibody
(OTI2D9)
NBP2-00776
Flow Cytometry CDK2 Antibody (OTI2D9)
Flow Cytometry CDK2 Antibody (OTI2D9)
Flow Cytometry CDK2 Antibody (OTI2D9)
(1 Review)
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TCEAL1 Antibody
(OTI2G10)
NBP2-46085
Immunohistochemistry TCEAL1 Antibody (OTI2G10)
Immunohistochemistry TCEAL1 Antibody (OTI2G10)
Immunohistochemistry TCEAL1 Antibody (OTI2G10)
Species: Hu
Applications: IHC, WB
D4S234E Antibody
(1C3)
H00027065-M01
Sandwich ELISA D4S234E Antibody (1C3)
Western Blot D4S234E Antibody (1C3)
Western Blot D4S234E Antibody (1C3)
(1 Publication)
Species: Hu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
PAK3 Antibody
[Unconjugated]
AF6897
Western Blot PAK3 Antibody [Unconjugated]
Immunohistochemistry PAK3 Antibody [Unconjugated]
Immunohistochemistry PAK3 Antibody [Unconjugated]
Species: Hu, Mu
Applications: IHC, Simple Western, WB
SLC12A9 Antibody
NBP2-68858
Immunohistochemistry-Paraffin SLC12A9 Antibody
Species: Hu
Applications: IHC, IHC-P
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
Western Blot p19ARF/CDKN2A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence p19ARF/CDKN2A Antibody - BSA Free
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
CDK4 Antibody
NBP1-31308
Immunocytochemistry/ Immunofluorescence CDK4 Antibody
Immunocytochemistry/ Immunofluorescence CDK4 Antibody
Western Blot CDK4 Antibody
(7 Publications)
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Cyclin D1 Antibody
(SPM587)
NBP2-32840
Western Blot Cyclin D1 Antibody (SPM587)
Flow Cytometry Cyclin D1 Antibody (SPM587)
Flow Cytometry Cyclin D1 Antibody (SPM587)
(1 Review)
(9 Publications)
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
PCNA Antibody
(PC10)
NB500-106
Immunocytochemistry/ Immunofluorescence PCNA Antibody (PC10)
Western Blot PCNA Antibody (PC10)
Flow Cytometry PCNA Antibody (PC10)
(5 Reviews)
(45 Publications)
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
RB1 Antibody
(607121) [Unconjugate
MAB6495
Western Blot RB1 Antibody (607121) [Unconjugated]
Immunocytochemistry RB1 Antibody (607121) [Unconjugated]
Simple Western RB1 Antibody (607121) [Unconjugated]
(4 Publications)
Species: Hu
Applications: ICC, Simple Western, WB

Publications for p27/Kip1 Antibody (FAB2256B) (0)

There are no publications for p27/Kip1 Antibody (FAB2256B).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for p27/Kip1 Antibody (FAB2256B) (0)

There are no reviews for p27/Kip1 Antibody (FAB2256B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for p27/Kip1 Antibody (FAB2256B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional p27/Kip1 Products

Research Areas for p27/Kip1 Antibody (FAB2256B)

Find related products by research area.

Blogs on p27/Kip1

There are no specific blogs for p27/Kip1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
PCNA Antibody (PC10)
NB500-106

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our p27/Kip1 Antibody (225501) [Biotin] and receive a gift card or discount.


Bioinformatics

Gene Symbol CDKN1B