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p130Cas/BCAR1 Antibody (OTI1A9) [Janelia Fluor® 585]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 585

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73181AF350
Alexa Fluor 405
NBP2-73181AF405
Alexa Fluor 488
NBP2-73181AF488
Alexa Fluor 532
NBP2-73181AF532
Alexa Fluor 594
NBP2-73181AF594
Alexa Fluor 647
NBP2-73181AF647
Alexa Fluor 700
NBP2-73181AF700
Alexa Fluor 750
NBP2-73181AF750
Allophycocyanin
NBP2-73181APC
Allophycocyanin/Cy7
NBP2-73181APCCY7
Biotin
NBP2-73181B
CoraFluor 1
NBP2-73181CL1
DyLight 350
NBP2-73181UV
DyLight 405
NBP2-73181V
DyLight 488
NBP2-73181G
DyLight 550
NBP2-73181R
DyLight 594
NBP2-73181DL594
DyLight 650
NBP2-73181C
DyLight 680
NBP2-73181FR
DyLight 755
NBP2-73181IR
FITC
NBP2-73181F
HRP
NBP2-73181H
Janelia Fluor 525
NBP2-73181JF525
Janelia Fluor 549
NBP2-73181JF549
Janelia Fluor 635
NBP2-73181JF635
Janelia Fluor 646
NBP2-73181JF646
Janelia Fluor 669
NBP2-73181JF669
PE
NBP2-73181PE
PE/Atto594
NBP2-73181PEATT594
PE/Cy5.5
NBP2-73181PECY55
PE/Cy7
NBP2-73181PECY7
PerCP
NBP2-73181PCP
Unconjugated
NBP2-00532
mFluor Violet 450 SE
NBP2-73181MFV450
mFluor Violet 500 SE
NBP2-73181MFV500
mFluor Violet 610 SE
NBP2-73181MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-00532
Azide and BSA Free
NBP2-73181
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p130Cas/BCAR1 Antibody (OTI1A9) [Janelia Fluor® 585] Summary

Immunogen
Full length human recombinant protein of human BCAR1 (NP_055382) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BCAR1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for p130Cas/BCAR1 Antibody (OTI1A9) [Janelia Fluor® 585]

  • BCAR1
  • breast cancer estrogen resistance 1
  • Cas scaffolding protein family member 1
  • CAS1
  • CASFLJ45059
  • CASS1
  • CASS1CRKASbreast cancer estrogen resistance protein 1
  • CRKAS
  • Crk-associated substrate p130Cas
  • CRK-associated substrate
  • FLJ12176
  • p130Cas

Background

BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells. Alternate names for BCAR1/p130CAS include breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, CAS scaffolding protein family member 1, CAS, and CRKAS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-73181JF585
p130Cas/BCAR1 Antibody (OTI1A9) [Janelia Fluor® 585]
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready

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Bioinformatics

Gene Symbol BCAR1