![Western Blot: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAR1.](http://images.novusbio.com/fullsize/p130Cas-BCAR1-Antibody-OTI1A9-Azide-and-BSA-Free-Western-Blot-NBP2-73181-img0002.jpg "Western Blot: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAR1.")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI1A9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human BCAR1 (NP_055382) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BCAR1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 93.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BCAR1 |
![Immunocytochemistry/Immunofluorescence: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1.](http://images.novusbio.com/fullsize/p130Cas-BCAR1-Antibody-OTI1A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73181-img0006.jpg "Immunocytochemistry/Immunofluorescence: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1.")
![Immunohistochemistry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BCAR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p130Cas-BCAR1-Antibody-OTI1A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73181-img0008.jpg "Immunohistochemistry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BCAR1 mouse monoclonal antibody.")
![Flow Cytometry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-BCAR1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/p130Cas-BCAR1-Antibody-OTI1A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73181-img0005.jpg "Flow Cytometry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-BCAR1 antibody, and then analyzed by flow cytometry.")
![Western Blot: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.](http://images.novusbio.com/fullsize/p130Cas-BCAR1-Antibody-OTI1A9-Azide-and-BSA-Free-Western-Blot-NBP2-73181-img0001.jpg "Western Blot: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.")
![Immunohistochemistry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BCAR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p130Cas-BCAR1-Antibody-OTI1A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73181-img0007.jpg "Immunohistochemistry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BCAR1 mouse monoclonal antibody.")
![Immunohistochemistry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p130Cas-BCAR1-Antibody-OTI1A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73181-img0009.jpg "Immunohistochemistry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody.")
![Flow Cytometry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Analysis of Hela cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/p130Cas-BCAR1-Antibody-OTI1A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73181-img0003.jpg "Flow Cytometry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Analysis of Hela cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Analysis of Jurkat cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/p130Cas-BCAR1-Antibody-OTI1A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73181-img0004.jpg "Flow Cytometry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Analysis of Jurkat cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")