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Human OXPAT ELISA Kit (Colorimetric)

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ELISA: Human OXPAT ELISA Kit (Colorimetric) [NBP3-39298] - Standard Curve Reference
ELISA: Human OXPAT ELISA Kit (Colorimetric) [NBP3-39298] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.065 ng/mL (example only; lot dependent)

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Human OXPAT ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.065 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PLIN5

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human OXPAT ELISA Kit (Colorimetric)

  • lipid storage droplet protein 5
  • LSDA5
  • LSDP5
  • MLDP
  • OXPAT
  • perilipin 5
  • perilipin-5

Background

OXPAT is a novel PAT-1 protein that is expressed in highly oxidative tissues. Lipid droplet proteins of the PAT family regulate cellular neutral lipid stores. OXPAT is induced by physiological (fasting), pathophysiological (insulin deficiency), pharmacological (peroxisome proliferator-activated receptor [PPAR] agonists), and genetic (muscle-specific PPAR alpha overexpression) disturbances that increase fatty acid utilization. It is suggested that OXPAT is a marker for PPAR activation and fatty acid oxidation. OXPAT may also contribute to adaptive responses to the fatty acid responsibility that accompanies fasting, insulin deficiency, and overnutrition responses that are defective in obesity and type 2 diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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OXPAT: Regulating Lipid Metabolism during Dietary Fluctuations
OXPAT is the mammalian form of the PAT (perilipin, adipophilin, and TIP47) gene family that consists of proteins associated with lipid droplets (LDs) in fat-storing adipocyte cells. They serve to protect LDs from natural lipases responsible for breaki...  Read full blog post.
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Bioinformatics

Gene Symbol PLIN5