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Human Osterix/Sp7 - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human Osterix/Sp7 - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39233

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ELISA: Human Osterix/Sp7 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39233] - Standard Curve Reference

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	ELISA
Suitable Sample Type	Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range	0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity	0.125 ng/mL (example only; lot dependent)

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Human Osterix/Sp7 - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description	The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time. Assay Length: 3 hours
Standard Curve Range	0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity	0.125 ng/mL (example only; lot dependent)
Assay Type	Sandwich ELISA
Inter-Assay	%CV < 12 (example only; lot dependent)
Intra-Assay	%CV < 10 (example only; lot dependent)
Sample Volume	100 uL
Kit Type	ELISA Kit (Colorimetric)
Gene	SP7

Applications/Dilutions

Dilutions

ELISA

Packaging, Storage & Formulations

Storage

Storage of components varies. See protocol for specific instructions.

Kit Components

Components

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard Diluent
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Alternate Names for Human Osterix/Sp7 - Ready-To-Use ELISA Kit (Colorimetric)

MGC126598
Osterix
OSX
OSXosterix
Sp7 transcription factor
Sp7
transcription factor Sp7
Zinc finger protein osterix

Background

SP7 is a C2H2-type zinc finger transcription factor of the SP gene family and a putative master regulator of bone cell differentiation (Gao et al., 2004 [PubMed 15474293]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human Osterix/Sp7
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NBP3-39233

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Publications for Osterix/Sp7 ELISA Kit (NBP3-39233) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Osterix/Sp7 ELISA Kit (NBP3-39233) (0)

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Customers Who Bought This Also Bought

Osteopontin/OPN was detected in immersion fixed C2C12 mouse myoblast cell line (left panel, positive stain) and mouse splenocytes (right panel, negative stain) using Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and secreted molecule. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Osteopontin/OPN Antibody [Unconjugated]
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Western blot shows lysates of Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Osterix/Sp7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Osterix/Sp7 Antibody (764704)
MAB7547

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Bioinformatics

Gene Symbol

SP7

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Human Osterix/Sp7 - Ready-To-Use ELISA Kit (Colorimetric)

Species Glossary

Images

Product Details

Order Details

Human Osterix/Sp7 - Ready-To-Use ELISA Kit (Colorimetric) Summary

Applications/Dilutions

Packaging, Storage & Formulations

Kit Components

Alternate Names for Human Osterix/Sp7 - Ready-To-Use ELISA Kit (Colorimetric)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Osterix/Sp7 ELISA Kit (NBP3-39233) (0)

Reviews for Osterix/Sp7 ELISA Kit (NBP3-39233) (0)

Product General Protocols

FAQs for Osterix/Sp7 ELISA Kit (NBP3-39233) (0)

Additional Osterix/Sp7 Products

Blogs on Osterix/Sp7

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Contact Information

Product PDFs

Review this Product

Bioinformatics