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Human Ornithine Carbamoyltransferase ELISA Kit (Colorimetric)

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ELISA: Human Ornithine Carbamoyltransferase ELISA Kit (Colorimetric) [NBP2-80259] - Samples were spiked with high concentrations of Human Ornithine Carbamoyltransferase and diluted with Reference Standard & Sample ...read more
ELISA: Human Ornithine Carbamoyltransferase ELISA Kit (Colorimetric) [NBP2-80259] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.469-30 ng/mL
Sensitivity
0.281 ng/mL

Order Details

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Human Ornithine Carbamoyltransferase ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human OCT in samples. No significant cross-reactivity or interference between Human OCT and analogues was observed.
Standard Curve Range
0.469-30 ng/mL
Sensitivity
0.281 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.64%
Intra-Assay
CV% < 5.2%
Spike Recovery
88-109%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
OTC

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25X)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Ornithine Carbamoyltransferase ELISA Kit (Colorimetric)

  • EC 2.1.3
  • EC 2.1.3.3
  • MGC129967
  • MGC129968
  • MGC138856
  • OCTD
  • Ornithine Carbamoyltransferase
  • ornithine carbamoyltransferase, mitochondrial
  • Ornithine Transcarbamylase
  • OTC
  • OTCase

Background

This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol OTC
Uniprot