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ORC1 Antibody

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Western Blot: ORC1 Antibody [NBP2-24600] - Analysis of ORC1L in Daudi cell lysate in the 1) absence and 2) presence of immunizing peptide and in 3) NIH 3T3 cell lysate using NBP2-24600. Goat anti-rabbit Ig HRP secondary ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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ORC1 Antibody Summary

Immunogen
A portion of amino acids 170 to 220 of human ORC1L was used as immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ORC1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml

Reactivity Notes

Canine (89%), Rat (84%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for ORC1 Antibody

  • HS
  • HSORC1
  • ORC1Lorigin recognition complex subunit 1
  • origin recognition complex, subunit 1 (yeast homolog)-like
  • origin recognition complex, subunit 1 homolog (S. cerevisiae)
  • origin recognition complex, subunit 1 homolog
  • origin recognition complex, subunit 1
  • origin recognition complex, subunit 1-like (yeast)
  • origin recognition complex, subunit 1-like
  • PARC1origin recognition complex 1
  • Replication control protein 1

Background

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ORC1