![Immunocytochemistry/Immunofluorescence: OGG1 Antibody (2B4) [NBP2-52724] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with OGG1 (2B4) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OGG1-Antibody-2B4-Immunocytochemistry-Immunofluorescence-NBP2-52724-img0002.jpg "Immunocytochemistry/Immunofluorescence: OGG1 Antibody (2B4) [NBP2-52724] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with OGG1 (2B4) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | 2B4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.9 mg/ml |
| Immunogen | Partial recombinant protein made to an internal sequence of the human OGG1 protein (between amino acids 100-300) [Uniprot: O15527] |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | OGG1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.9 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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You down with Ogg? Yeah, you know me 8-hydroxyguanine is a form of oxidative DNA damage where free radicals cause G:C to T:A transversions within the backbone. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine mutations. One of these enzymes,... Read full blog post. |
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| Gene Symbol | OGG1 |
![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) [NBP2-52724] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells renal cell carcinoma.](http://images.novusbio.com/fullsize/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0006.jpg "Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) [NBP2-52724] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells renal cell carcinoma.")
![Flow (Intracellular): OGG1 Antibody (2B4) [NBP2-52724] - An intracellular stain was performed on HeLa cells with OGG1 (2B4) antibody NBP2-52724 (Blue) and a matched isotype control NBP2-27231 (Orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/OGG1-Antibody-2B4-Flow-Intracellular-NBP2-52724-img0001.jpg "Flow (Intracellular): OGG1 Antibody (2B4) [NBP2-52724] - An intracellular stain was performed on HeLa cells with OGG1 (2B4) antibody NBP2-52724 (Blue) and a matched isotype control NBP2-27231 (Orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).")
![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) [NBP2-52724] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells rectum adenocarcinoma.](http://images.novusbio.com/fullsize/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0004.jpg "Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) [NBP2-52724] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells rectum adenocarcinoma.")
![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) [NBP2-52724] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells endometrial carcinoma.](http://images.novusbio.com/fullsize/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0005.jpg "Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) [NBP2-52724] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells endometrial carcinoma.")
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