8-hydroxyguanine is a form of oxidative DNA damage where free radicals cause G:C to T:A transversions within the backbone. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine mutations. One of these enzymes, Ogg1, was found to have both functional yeast (yOgg1) and human (hOgg1) homologues. These hOgg1 proteins efficiently release the 8-hydroxyguanine through cleavage. Genetic backgrounds in control of repair of damaged DNA are involved in the susceptibility of affected cells to cancer development. Recent work with the Ogg1 antibody has helped establish the role of a p53-mediated cascade in activation of melanocortin 1 receptor (MC1R) downstream signaling and the malignant transformation of melanocytes in UV damage and oxidative stress conditions (1).
Immunohistochemistry-Frozen: Ogg1 Antibody
Other Ogg1 antibody-based UV-damage studies found that vitamin B (nicotinamide) enhances the repair of UV-induced DNA damage in both human keratinocyte and ex vivo skin test models (2). Researchers used the Ogg1 antibody to confirm that the combinatory effect of Ogg1 down regulation and accumulation of reactive oxygen species (ROS) in basal cell carcinoma (BCC) is to drive transformation and aggressive tumor progression (3). Co-localization of Ogg1 and its bound proteins using Ogg1 antibody showed a unique association with the RAD52 protein that indicates cooperation between the two components to repair DNA damage and enhance cellular resistance to further damage (4). Recent fertility studies using the Ogg1 antibody have found that mature spermatozoa have a limited capacity to mount their DNA repair mechanisms in response to oxidative stress, possibly protecting an embryo from paternally mediated genetic damage (5).
Novus Biologicals offers various Ogg1 reagents for your research needs including:
