Home » NuMA » NuMA Antibodies » NuMA Antibody (SPM300) - Azide and BSA Free
Novus Biologicals products are now on bio-techne.com

NuMA Antibody (SPM300) - Azide and BSA Free

 Submit a Review

Images

 
There are currently no images for NuMA Antibody (NBP3-11547).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, ICC/IF
Clone
SPM300
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45227-0.1mg
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

NuMA Antibody (SPM300) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by PEG precipitation prepared in 10 mM PBS WITHOUT BSA & azide.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Colon carcinoma 174T cells
Localization
Nuclear
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NUMA1
Purity
PEG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
228 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
PEG purified

Alternate Names for NuMA Antibody (SPM300) - Azide and BSA Free

  • centrophilin stabilizes mitotic spindle in mitotic cells
  • NMP-22
  • nuclear mitotic apparatus protein 1
  • NuMA protein
  • NuMA
  • NUMA1
  • SP-H Antigen
  • structural nuclear protein

Background

NuMA (nuclear mitotic apparatus) is a long coiled-coil protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning. Therefore, this antibody is useful as a cell cycle marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

GPSM2 Antibody
NBP1-53125
Immunohistochemistry GPSM2 Antibody
Western Blot GPSM2 Antibody
Immunocytochemistry/ Immunofluorescence GPSM2 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NPM1 Antibody -
BSA Free
NB110-61646
Immunocytochemistry/ Immunofluorescence NPM1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence NPM1 Antibody - BSA Free
Western Blot NPM1 Antibody - BSA Free
(2 Reviews)
(4 Publications)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Eg5 Antibody
NB500-181
Western Blot Eg5 Antibody
Immunocytochemistry/ Immunofluorescence Eg5 Antibody
Western Blot Eg5 Antibody
(2 Reviews)
(21 Publications)
Species: Dr, Hu, Ma, Mu, Pl, Po, Rt
Applications: IB, ICC/IF, IP, WB
PARP Antibody
(194C1439) - Cleaved ...
NBP2-27335
Immunocytochemistry/ Immunofluorescence PARP Antibody (194C1439) - Cleaved - Azide and BSA Free
Western Blot PARP Antibody (194C1439) - Cleaved - Azide and BSA Free
Western Blot PARP Antibody (194C1439) - Cleaved - Azide and BSA Free
(1 Review)
(9 Publications)
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, WB
RAR alpha/NR1B1
Antibody (763)
NB200-322
Western Blot RAR alpha/NR1B1 Antibody (763)
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, WB
Caspase-3 Antibody
[Unconjugated] - Acti ...
AF835
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
(7 Reviews)
(389 Publications)
Species: Hu, Mu
Applications: ICC, IHC
PML Protein
Antibody - BSA Free
NB100-59787
Western Blot PML Protein Antibody - BSA Free
Immunohistochemistry PML Protein Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence PML Protein Antibody - BSA Free
(1 Review)
(42 Publications)
Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, IP, KD, PLA, WB
PLZF Antibody
[Unconjugated]
AF2944
Western Blot PLZF Antibody [Unconjugated]
Simple Western PLZF Antibody [Unconjugated]
Western Blot PLZF Antibody [Unconjugated]
(45 Publications)
Species: Hu
Applications: Simple Western, WB
Tankyrase 1
Antibody
AF7116
Western Blot Tankyrase 1 Antibody
(1 Publication)
Species: Hu
Applications: WB
EPB41 Antibody
(3D9)
H00002035-M01
Western Blot EPB41 Antibody (3D9)
Immunocytochemistry/ Immunofluorescence EPB41 Antibody (3D9)
Immunohistochemistry-Paraffin EPB41 Antibody (3D9)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
CDC2/CDK1 Antibody
NBP1-85729
Immunohistochemistry-Paraffin CDC2/CDK1 Antibody
Western Blot CDC2/CDK1 Antibody
Western Blot CDC2/CDK1 Antibody
(5 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
KIFC1 Antibody
NB100-40844
Immunohistochemistry-Paraffin KIFC1 Antibody
Immunoprecipitation KIFC1 Antibody
(5 Publications)
Species: Hu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB
GAS41 Antibody
NBP3-38243
Western Blot GAS41 Antibody
Immunohistochemistry GAS41 Antibody
Immunocytochemistry/ Immunofluorescence GAS41 Antibody
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Collagen XI alpha
2 Antibody (473)
NBP2-43728
Immunocytochemistry/ Immunofluorescence Collagen XI alpha 2 Antibody (473)
Western Blot Collagen XI alpha 2 Antibody (473)
Western Blot Collagen XI alpha 2 Antibody (473)
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
STAT5b Antibody
[Unconjugated]
AF1584
Simple Western STAT5b Antibody [Unconjugated]
Knockout Validated STAT5b Antibody [Unconjugated]
Immunocytochemistry STAT5b Antibody [Unconjugated]
(1 Review)
(8 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IP, ICFlow, KO, Simple Western, WB
Ran Antibody
(8D1A6) - BSA Free
NBP2-61832
ELISA Ran Antibody (8D1A6) - BSA Free
Flow Cytometry Ran Antibody (8D1A6) - BSA Free
Immunocytochemistry/ Immunofluorescence Ran Antibody (8D1A6) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
RCC1 Antibody
NBP1-85637
Immunocytochemistry/ Immunofluorescence RCC1 Antibody
Western Blot RCC1 Antibody
Immunohistochemistry-Paraffin RCC1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
TPX2 Antibody
NB500-179
Flow Cytometry TPX2 Antibody
Simple Western TPX2 Antibody
Immunocytochemistry/ Immunofluorescence TPX2 Antibody
(1 Review)
(47 Publications)
Species: Hu, Ma, Mu, Po, Rt
Applications: Flow, ICC/IF, IP, KD, Simple Western, WB

Publications for NuMA Antibody (NBP3-11547) (0)

There are no publications for NuMA Antibody (NBP3-11547).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NuMA Antibody (NBP3-11547) (0)

There are no reviews for NuMA Antibody (NBP3-11547). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for NuMA Antibody (NBP3-11547) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional NuMA Products

Research Areas for NuMA Antibody (NBP3-11547)

Find related products by research area.

Blogs on NuMA.

Check out the latest blog posts on NuMA.
NuMA: The Key to Asymmetric Cell Division
Nuclear Mitotic Apparatus protein (NuMA) is a cell cycle-related protein that acts as an organizer of the mitotic spindle during mitosis. It may be involved in coordinating the alignment of the mitotic spindle to the cellular polarity axis, which is a...  Read full blog post.
Nucleus and Mitotic Apparatus (NuMA) Protein in Cell Cycle Regulation
NuMA, the major protein of the "Nucleus and Mitotic Apparatus", is a structural protein in vertebrates involved in cell cycle regulation. It localizes to the nucleus during interphase, and accumulates at the spindle poles during mitosis and NuMA has b...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NuMA Antibody (SPM300) - Azide and BSA Free and receive a gift card or discount.


Bioinformatics

Gene Symbol NUMA1