NKX6.1 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39‑46 kDa member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a beta -cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces beta -cell mitosis. Human NKX6.1 is 367 amino acids (aa) in length. It contains a repressor region composed of Pro and Ala (aa 136‑173), a DNA binding homeobox (aa 236‑295) and a Glu:Asp‑rich transactivation domain (aa 305‑337). Over aa 1‑120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-P, Western Blot.

Showing Publications 1 - 10 of 19. Show All 19 Publications. Collapse Publications.

Publications using AF5857	Applications	Species
Xisheng Li, Zhangjing Ma, Kathy O. Lui A differentiation protocol for the generation of pancreatic beta-like cells from human embryonic stem cells STAR Protocols 9/1/2023 [PMID: 37392391]
C Uhlemeyer, N Müller, M Rieck, J Kuboth, C Schlegel, K Grie beta , TF Dorweiler, S Heiduschka, J Eckel, M Roden, E Lammert, M Stoffel, BF Belgardt Selective ablation of P53 in pancreatic beta cells fails to ameliorate glucose metabolism in genetic, dietary and pharmacological models of diabetes mellitus Molecular Metabolism, 2022-12-05;67(0):101650. 2022-12-05 [PMID: 36470401] (IHC, Mouse)	IHC	Mouse
WM Seo, J Yoon, JH Lee, Y Lee, H Lee, D Geum, W Sun, MR Song Modeling axonal regeneration by changing cytoskeletal dynamics in stem cell-derived motor nerve organoids Scientific Reports, 2022-02-08;12(1):2082. 2022-02-08 [PMID: 35136073] (IHC, Human)	IHC	Human
NF Chhabra, AL Amend, A Bastidas-P, S Sabrautzki, M Tarquis-Me, S Sachs, M Rubey, B Lorenz-Dep, A Feuchtinge, M Bakhti, H Lickert, GKH Przemeck, M Hrab? de A A point mutation in the Pdia6 gene results in loss of pancreatic beta-cell identity causing overt diabetes Molecular Metabolism, 2021-09-04;0(0):101334. 2021-09-04 [PMID: 34487921] (IHC, Mouse)	IHC	Mouse
R Simone, F Javad, W Emmett, OG Wilkins, FL Almeida, N Barahona-T, J Zareba-Pas, M Ehteramyan, P Zuccotti, A Modelska, K Siva, GS Virdi, JS Mitchell, J Harley, VA Kay, G Hondhamuni, D Trabzuni, M Ryten, S Wray, E Preza, DA Kia, A Pittman, R Ferrari, C Manzoni, A Lees, JA Hardy, MA Denti, A Quattrone, R Patani, P Svenningss, TT Warner, V Plagnol, J Ule, R de Silva MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration Nature, 2021-05-19;0(0):. 2021-05-19 [PMID: 34012113] (ICC, Human)	ICC	Human
Ingo Burtscher, Marta Tarquis-Medina, Ciro Salinno, Silvia Schirge, Julia Beckenbauer, Mostafa Bakhti et al. Generation of a Novel Nkx6-1 Venus Fusion Reporter Mouse Line International Journal of Molecular Sciences 3/26/2021 [PMID: 33810480]
Reena Singh, Louise Cottle, Thomas Loudovaris, Di Xiao, Pengyi Yang, Helen E. Thomas et al. Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix Stem Cells Translational Medicine 3/1/2021 [PMID: 33145960]
Hsin-Hua Chung, Chun-Te Lee, Je-Ming Hu, Yu-Ching Chou, Ya-Wen Lin, Yu-Lueng Shih NKX6.1 Represses Tumorigenesis, Metastasis, and Chemoresistance in Colorectal Cancer International Journal of Molecular Sciences 7/19/2020 [PMID: 32707737]
V Ilievski, PT Toth, K Valyi-Nagy, T Valyi-Nagy, SJ Green, RS Marattil, HW Aljewari, B Wicksteed, NM O'Brien-Si, EC Reynolds, BT Layden, TG Unterman, K Watanabe Identification of a periodontal pathogen and bihormonal cells in pancreatic islets of humans and a mouse model of periodontitis Sci Rep, 2020-06-19;10(1):9976. 2020-06-19 [PMID: 32561770] (ICC, Human, Mouse)	ICC	Human, Mouse
Mahaddalkar PU, Scheibner K, Pfluger S et al. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm Nature Biotechnology 9/1/2020 [PMID: 32341565]
Mahaddalkar PU, Scheibner K, Pfluger S et al. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm Nature Biotechnology 2020-09-01 [PMID: 32341565]
Seokho Kim, Robert L. Whitener, Heshan Peiris, Xueying Gu, Charles A. Chang, Jonathan Y. Lam et al. Molecular and genetic regulation of pig pancreatic islet cell development Development 1/1/2020 [PMID: 32108026]
Francesco Volta, M. Julia Scerbo, Anett Seelig, Robert Wagner, Nils O’Brien, Felicia Gerst et al. Glucose homeostasis is regulated by pancreatic beta -cell cilia via endosomal EphA-processing Nature Communications 12/12/2019 [PMID: 31831727]
U Diekmann, H Wolling, R Dettmer, I Niwolik, O Naujok, FFR Buettner Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture Sci Rep, 2019-01-30;9(1):996. 2019-01-30 [PMID: 30700818] (ICC, Human)	ICC	Human
B Memon, M Karam, S Al-Khawaga, EM Abdelalim Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1 Stem Cell Res Ther, 2018-01-23;9(1):15. 2018-01-23 [PMID: 29361979] (Flow Cytometry, Human)	Flow Cytometry	Human
A. S. Axelsson, T. Mahdi, H. A. Nenonen, T. Singh, S. Hänzelmann, A. Wendt et al. Sox5 regulates beta-cell phenotype and is reduced in type 2 diabetes Nature Communications 6/6/2017 [PMID: 28585545]
Identification of proliferative and mature ?-cells in the islets of Langerhans Nature, 2016-07-11;535(7612):430-4. 2016-07-11 [PMID: 27398620] (Flow Cytometry, IHC-P, Western Blot, Human)	Flow Cytometry, IHC-P, Western Blot	Human
Masjkur J, Arps-Forker C, Poser S, Nikolakopoulou P, Toutouna L, Chenna R, Chavakis T, Chatzigeorgiou A, Chen L, Dubrovska A, Choudhary P, Uphues I, Mark M, Bornstein S, Androutsellis-Theotokis A Hes3 is expressed in the adult pancreatic islet and regulates gene expression, cell growth, and insulin release. J Biol Chem, 2014-11-04;289(51):35503-16. 2014-11-04 [PMID: 25371201] (ICC, Human)	ICC	Human
Latreille M, Hausser J, Stutzer I, Zhang Q, Hastoy B, Gargani S, Kerr-Conte J, Pattou F, Zavolan M, Esguerra J, Eliasson L, Rulicke T, Rorsman P, Stoffel M MicroRNA-7a regulates pancreatic beta cell function. J Clin Invest, 2014-05-01;124(6):2722-35. 2014-05-01 [PMID: 24789908] (IHC, Mouse)	IHC	Mouse
Show All 19 Publications. Collapse Publications.

Array AF5857

• NKX6.1 Antibodies
• NKX6.1 ELISA Kits
• NKX6.1 Lysate
• NKX6.1 Protein