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NKX2.2 Antibody (NX2/1523) - Azide and BSA Free

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Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/1523) - Azide and BSA Free [NBP2-54328] - Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with NKX2.2 Monoclonal Antibody (NX2/1523).
Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/1523) - Azide and BSA Free [NBP2-54328] - Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with NKX2.2 Monoclonal Antibody (NX2/1523).
Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/1523) - Azide and BSA Free [NBP2-54328] - Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with NKX2.2 Monoclonal Antibody (NX2/1523).
Protein Array: NKX2.2 Antibody (NX2/1523) - Azide and BSA Free [NBP2-54328] - Analysis of Protein Array containing >19,000 full-length human proteins using NKX2.2 Antibody (NX2/1523) Z- and S- Score: The Z-score ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, MA
Clone
NX2/1523
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54328AF350
Alexa Fluor 405
NBP2-54328AF405
Alexa Fluor 488
NBP2-54328AF488
Alexa Fluor 532
NBP2-54328AF532
Alexa Fluor 594
NBP2-54328AF594
Alexa Fluor 647
NBP2-54328AF647
Alexa Fluor 700
NBP2-54328AF700
Alexa Fluor 750
NBP2-54328AF750
Biotin
NBP2-54328B
DyLight 350
NBP2-54328UV
DyLight 405
NBP2-54328V
DyLight 488
NBP2-54328G
DyLight 550
NBP2-54328R
DyLight 594
NBP2-54328DL594
DyLight 650
NBP2-54328C
DyLight 680
NBP2-54328FR
DyLight 755
NBP2-54328IR
FITC
NBP2-54328F
HRP
NBP2-54328H
Janelia Fluor 549
NBP2-54328JF549
Janelia Fluor 646
NBP2-54328JF646
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NKX2.2 Antibody (NX2/1523) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53200).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant fragment (around aa1-119) of human NKX2.2 protein (exact sequence is proprietary) (Uniprot: O95096)
Localization
Nuclear
Marker
Neuroendocrine & Ewing's Sarcoma Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NKX2-2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for NKX2.2 Antibody (NX2/1523) - Azide and BSA Free

  • Homeobox protein NK-2 homolog B
  • NK-2 (Drosophila) homolog B
  • NK2 homeobox 2
  • NK2 transcription factor related, locus 2 (Drosophila)
  • NK2 transcription factor related, locus 2
  • NK2 transcription factor-like protein B
  • NKX2.2
  • NKX2.2homeobox protein Nkx-2.2
  • NKX2B
  • NKX2BNK-2 homolog B

Background

Nkx2.2, or Homeobox protein Nkx-2.2, consists of a 273 amino acid isoform that is 30 kDa, and is involved in controlling and maintaining the expression of genes within pancreatic endocrine cells and axons. Current research is being conducted on the relationship between Nkx2.2 and a variety of diseases and disorders, including glioma, neuronitis, maturity-onset diabetes, pancreatitis, Partington syndrome, sarcoma, gastrointestinal neuroendocrine tumor, acute lymphoblastic leukemia, multiple sclerosis, and carcinoid tumors. The protein has been linked to the Wnt, Hedgehog, and notch pathways, as well as regulation of beta-cell development and gene expression. NKx2.2 interacts with several other proteins, including OLIG2, HDAC1, SIN3A, FOXA2, and FOXO1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NKX2-2