Novus Biologicals products are now on bio-techne.com

NKCC2/SLC12A1 Recombinant Protein Antigen

Images

 
There are currently no images for NKCC2/SLC12A1 Protein (NBP1-80993PEP).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

NKCC2/SLC12A1 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human SLC12A1.

Source: E. coli

Amino Acid Sequence: MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSI

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
SLC12A1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-80993.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

For further blocking peptide related information and a protocol, click here.

Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for NKCC2/SLC12A1 Recombinant Protein Antigen

  • BSC1
  • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2
  • Kidney-specific Na-K-Cl symporter
  • MGC48843
  • NKCC2
  • NKCC2A variant A
  • NKCC2Na-K-2Cl cotransporter
  • SLC12A1
  • solute carrier family 12 (sodium/potassium/chloride transporters), member 1
  • solute carrier family 12 member 1

Background

The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-44270
Species: Ca, Hu, Mu, Rt
Applications: EM, ICC/IF, IHC, IHC-P, WB
NBP1-52148
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
NBP1-82874
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
NB110-74682
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-82574
Species: Hu, Mu, Po
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, PAGE, WB
NBP1-88109
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NBP1-82016
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-10348
Species: Hu
Applications: WB
NBP1-48021
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MAB4447
Species: Hu
Applications: IHC, WB
NB600-749
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
AF009
Species: Hu
Applications: IHC, WB
MAB6495
Species: Hu
Applications: ICC, Simple Western, WB
NBP1-30027
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF5175
Species: Mu
Applications: IHC, Simple Western, WB
NBP2-49101
Species: Hu
Applications: IHC, IHC-P
H00009943-M16
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NBP1-80993PEP
Species: Hu
Applications: AC

Publications for NKCC2/SLC12A1 Protein (NBP1-80993PEP) (0)

There are no publications for NKCC2/SLC12A1 Protein (NBP1-80993PEP).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NKCC2/SLC12A1 Protein (NBP1-80993PEP) (0)

There are no reviews for NKCC2/SLC12A1 Protein (NBP1-80993PEP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for NKCC2/SLC12A1 Protein (NBP1-80993PEP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NKCC2/SLC12A1 Products

Research Areas for NKCC2/SLC12A1 Protein (NBP1-80993PEP)

Find related products by research area.

Blogs on NKCC2/SLC12A1

There are no specific blogs for NKCC2/SLC12A1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

The molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol)

As an example, if the molecular weight of a compound is 197.13 g/mol and the desired concentration is 10 mM for 10 ml of water based stock solution, the required mass would be = 19.713 (value determined by this calculator).

=
x
x
g/mol

Review this Product

Be the first to review our NKCC2/SLC12A1 Recombinant Protein Antigen and receive a gift card or discount.

Bioinformatics

Gene Symbol SLC12A1