Home » NKCC1/SLC12A2 » NKCC1 Antibodies » NKCC1/SLC12A2 Antibody (915911) [Unconjugated]

NKCC1/SLC12A2 Antibody (915911) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP was stained with either (A) Mouse Anti-Human NKCC1/SLC12A2 Monoclonal Antibody (Catalog # MAB8528) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP was stained with either (A) Mouse Anti-Human NKCC1/SLC12A2 Monoclonal Antibody (Catalog # MAB8528) or (B) Mouse IgG2B Flow Cytometry ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
915911
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB8528U-100UG
Alexa Fluor 405
FAB8528V-100UG
Alexa Fluor 488
FAB8528G-100UG
Alexa Fluor 532
FAB8528X
Alexa Fluor 594
FAB8528T-100UG
Alexa Fluor 647
FAB8528R-100UG
Alexa Fluor 700
FAB8528N-100UG
Alexa Fluor 750
FAB8528S-100UG
Allophycocyanin
FAB8528A-025
Allophycocyanin/Cy7
FAB8528APCCY7
Biotin
FAB8528B
CoraFluor 1
FAB8528CL1
DyLight 350
FAB8528D
DyLight 405
FAB8528E
DyLight 488
FAB8528K
DyLight 550
FAB8528L
DyLight 594
FAB8528M
DyLight 650
FAB8528W
DyLight 680
FAB8528Y
DyLight 755
FAB8528Z
FITC
FAB8528F
HRP
FAB8528H
Janelia Fluor 525
FAB8528JF525
Janelia Fluor 549
FAB8528I
Janelia Fluor 646
FAB8528J
Janelia Fluor 669
FAB8528JF669
PE
FAB8528P
PE/Atto594
FAB8528PEATT594
PE/Cy5.5
FAB8528PECY55
PE/Cy7
FAB8528PECY7
PerCP
FAB8528C
mFluor Violet 450 SE
FAB8528MFV450
mFluor Violet 500 SE
FAB8528MFV500
mFluor Violet 610 SE
FAB8528MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8528-SP
Carrier Free
FAB8528A-025
Carrier Free
FAB8528S-100UG
Carrier Free
FAB8528R-100UG
Carrier Free
FAB8528N-100UG
Carrier Free
FAB8528G-100UG
Carrier Free
FAB8528V-100UG
Carrier Free
FAB8528U-100UG
Carrier Free
FAB8528T-100UG
Novus Biologicals offers 100% Guarantee

NKCC1/SLC12A2 Antibody (915911) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human NKCC1/SLC12A2
Met1-Ser1212
Accession # P55011
Specificity
Stains NKCC1/SLC12A2 transfectants but not irrelevant transfectants in flow cytometry.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SLC12A2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKCC1/SLC12A2 Antibody (915911) [Unconjugated]

  • Basolateral Na-K-Cl symporter
  • BSC
  • BSC2
  • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
  • NKCC1
  • NKCC1MGC104233
  • SLC12A2
  • solute carrier family 12 (sodium/potassium/chloride transporters), member 2
  • solute carrier family 12 member 2

Background

Solute Carrier, family 12 (potassium/chloride transporter), member 2 (SLC12A2) encodes NKCC1, which mediates sodium and chloride transport and reabsorption. It is a membrane protein that is important in maintaining proper ionic balance and cell volume. In contrast to the renal-specific sodium/potassium/chloride cotransporter SLC12A1, SLC12A2 is expressed in many tissues, including the basolateral membrane of secretory epithelia. It is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for SLC12A2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB8528
NKCC1/SLC12A2 Antibody (915911) [Unconjugated]
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Bioinformatics

Gene Symbol SLC12A2
Uniprot