Niemann-Pick type C1 Like-1 Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, MS, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

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Niemann-Pick type C1 Like-1 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
A synthetic peptide made to an internal region of rat Niemann-Pick type C1 Like-1 (between residues 1000-1100). [UniProt# Q6T3U3]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPC1L1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Electron Microscopy
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Niemann-Pick type C1 Like-1 Antibody [CoraFluor™ 1]

  • Niemann-Pick C1-like protein 1
  • NPC1 (Niemann-Pick disease, type C1, gene)-like 1
  • NPC11L1

Background

Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NPC1L1