NHE3/SLC9A3 [p Ser605] Antibody (10A8)

Na+/H+ exchanger (NHE) family members are integral membrane proteins which play key role in catalyzing the extrusion of intracellular proton (H+) ions in exchange for extracellular sodium (Na+) ions for regulation of cellular pH as well as transepithelial ion/water transport. NHE3 or SLC9A3 is one of the best characterized NHEs that is a major proton extruding system driven by inward Na+ ion chemical gradient and is implicated in pH regulation to eliminate acids generated during metabolism or to tackle any potential adverse conditions in tissue microenvironment. NHE3 exists as a multi-pass membrane protein in cell membranes and is expressed most abundantly in colon and small intestine, followed by kidney and stomach, as well as in ventrolateral region of the medulla oblongata. In intestinal epithelial cells, it localizes to the ileal brush border and SGK1 mediated phosphorylation at Ser-661 has been shown to be associated with its enhanced cell membrane localization. NHE3 binds to SLC9A3R1 and SLC9A3R2, and interacts with CHP1, CHP2, SHANK2 PDZD3 and (interactions may decrease in response to elevated calcium ion levels). NHE3 is regulated by several hormones, neurotransmitters, and associated signaling systems such as cAMP, cGMP, and elevated intracellular calcium via mechanisms that are largely partially understood. NHE3 knock-out mice suffers from chronic diarrhea and altered salt as well as water homeostasis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Rat, Opossum, Porcine, Primate.

We have publications tested in 2 applications: SDS-Page, WB.

Showing Publications 1 - 5 of 5.

Publications using NB110-74678	Applications	Species
Klinger S Segment specific effects of resveratrol on porcine small intestinal dipeptide absorption depend on the mucosal pH and are due to different mechanisms: Potential roles of different transport proteins and protein kinases J. Nutr. Biochem. 2020-07-29 [PMID: 32738496] (SDS-Page, WB, Porcine)	SDS-Page, WB	Porcine
Klinger S, Breves G. Resveratrol Inhibits Porcine Intestinal Glucose and Alanine Transport: Potential Roles of Na+/K+-ATPase Activity, Protein Kinase A, AMP-Activated Protein Kinase and the Association of Selected Nutrient Transport Proteins with Detergent Resistant Membranes Nutrients. 2018-03-03 [PMID: 29510506] (WB, Porcine)	WB	Porcine
a DW, Steinmetz AG, Kocinsky HS. NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase. Am J Physiol Renal Physiol;298(3):F745-53. 2010-03-01 [PMID: 20015946]
Kocinsky HS, Dynia DW, Wang T, Aronson PS. NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. Am J Physiol Renal Physiol;293(1):F212-8. 2007-07-01 [PMID: 17409282] (WB, Opossum, Rat)	WB	Opossum, Rat
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS. Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites. Am J Physiol Renal Physiol;289(2):F249-58. 2005-08-01 [PMID: 15687252] (WB, Opossum, Primate)	WB	Opossum, Primate

Array NB110-74678

• NHE3/SLC9A3 Antibodies
• NHE3/SLC9A3 Proteins