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NF-M Antibody (MCA-3H11)

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Western Blot: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of neuronal tissue lysates using mouse NF-M mAb, dilution 1:10,000 (Green): [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow ...read more
Immunohistochemistry Free-Floating: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of adult rat frontal cortex section stained with mouse neurofilament NF-M mAb, dilution 1:5,000 (Green), and costained with chicken ...read more
Western Blot: Neurofilament NF-M Antibody (MCA-3H11) [NBP2-31206] - Cytoskeletal homogenate of rat spinal cord was separated by 7.5% SDS-PAGE. Lane A: Western blot analysis of Neurofilament NF-M with NBP2-31206. Lane B: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Eq, FeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC
Clone
MCA-3H11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

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NF-M Antibody (MCA-3H11) Summary

Immunogen
Recombinant fusion protein containing the extreme C-terminus of rat NF-M (KE segment), amino acids 677-845, expressed in and purified from E. coli. The so-called KE segment, is highly conserved between NF-M molecules from different species.
Predicted Species
Feline (98%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NEFM
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry Free-Floating 1:5000
  • Immunohistochemistry 1:500
  • Western Blot 1:5000
Application Notes
NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at ~145-160 kDa, with some variability across species. Users should titrate the antibody to determine the optimal concentration for their experimental system.
Theoretical MW
145-160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% glycerol
Preservative
0.035% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified

Alternate Names for NF-M Antibody (MCA-3H11)

  • NEF3
  • NEF3neurofilament medium polypeptide
  • NEFM
  • Neurofilament 3
  • neurofilament, medium polypeptide 150kDa
  • neurofilament, medium polypeptide
  • neurofilament-3 (150 kD medium)
  • NFM
  • NF-M
  • NF-M160 kDa neurofilament protein
  • NFMNeurofilament triplet M protein

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NEFM
Uniprot