NF-M Antibody (MCA-3H11) Summary
Immunogen |
Recombinant fusion protein containing the extreme C-terminus of rat NF-M (KE segment), amino acids 677-845, expressed in and purified from E. coli. The so-called KE segment, is highly conserved between NF-M molecules from different species. |
Predicted Species |
Feline (98%). Backed by our 100% Guarantee. |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
NEFM |
Purity |
Unpurified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:500
- Immunohistochemistry Free-Floating 1:5000
- Immunohistochemistry 1:500
- Western Blot 1:5000
|
Application Notes |
NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at ~145-160 kDa, with some variability across species. Users should titrate the antibody to determine the optimal concentration for their experimental system. |
Theoretical MW |
145-160 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
50% PBS, 50% glycerol |
Preservative |
0.035% Sodium Azide |
Concentration |
This product is unpurified. The exact concentration of antibody is not quantifiable. |
Purity |
Unpurified |
Alternate Names for NF-M Antibody (MCA-3H11)
Background
Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.
Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
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