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NF-M Antibody (CL2697)

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Western Blot: NF-M Antibody (2697) [NBP2-46619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human liver shows absence of immunoreactivity (negative control).
Western Blot: NF-M Antibody (2697) [NBP2-46619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of rat cerebral cortex shows strong immunoreactivity in neuronal processes.
Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of mouse cerebral cortex shows strong immunoreactivity in neuronal processes.
Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human cerebral cortex shows strong positivity in neural fibers.
Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of rat striatum shows strong positivity in neural fibers.
Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of mouse trigeminal ganglion shows strong positivity in primary sensory neurons.
Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neuronal processes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL2697
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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NF-M Antibody (CL2697) Summary

Immunogen
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence YIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEEASLVKVELDKKVQSLQDEVAFLRSNH
Epitope
SDHLEEDIHRLKERF
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NEFM
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for NF-M Antibody (CL2697)

  • NEF3
  • NEF3neurofilament medium polypeptide
  • NEFM
  • Neurofilament 3
  • neurofilament, medium polypeptide 150kDa
  • neurofilament, medium polypeptide
  • neurofilament-3 (150 kD medium)
  • NFM
  • NF-M
  • NF-M160 kDa neurofilament protein
  • NFMNeurofilament triplet M protein

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol

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Bioinformatics

Gene Symbol NEFM
Entrez
Uniprot