![Western Blot: NF-L Antibody (3L9Y10) [NBP3-15874] - Western blot analysis of extracts of SH-SY5Y cells, using NF-L antibody (NBP3-15874) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/NF-L-Antibody-3L9Y10-Western-Blot-NBP3-15874-img0006.jpg "Western Blot: NF-L Antibody (3L9Y10) [NBP3-15874] - Western blot analysis of extracts of SH-SY5Y cells, using NF-L antibody (NBP3-15874) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 3L9Y10 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human NF-L (NP_006149.2). SSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVT |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | NEFL |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.05% Proclin 300 |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for NF-L Antibody (NBP3-15874)Find related products by research area.
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| Gene Symbol | NEFL |
![Immunohistochemistry: NF-L Antibody (3L9Y10) [NBP3-15874] - Immunofluorescence analysis of mouse brain using NF-L Rabbit mAb (NBP3-15874) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-3L9Y10-Immunohistochemistry-NBP3-15874-img0004.jpg "Immunohistochemistry: NF-L Antibody (3L9Y10) [NBP3-15874] - Immunofluorescence analysis of mouse brain using NF-L Rabbit mAb (NBP3-15874) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Western Blot: NF-L Antibody (3L9Y10) [NBP3-15874] - Western blot analysis of extracts of various cell lines, using NF-L antibody (NBP3-15874) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/NF-L-Antibody-3L9Y10-Western-Blot-NBP3-15874-img0005.jpg "Western Blot: NF-L Antibody (3L9Y10) [NBP3-15874] - Western blot analysis of extracts of various cell lines, using NF-L antibody (NBP3-15874) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: NF-L Antibody (3L9Y10) [NBP3-15874] - Mouse brain using NF-L Rabbit mAb (NBP3-15874) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NF-L-Antibody-3L9Y10-Immunohistochemistry-Paraffin-NBP3-15874-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (3L9Y10) [NBP3-15874] - Mouse brain using NF-L Rabbit mAb (NBP3-15874) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: NF-L Antibody (3L9Y10) [NBP3-15874] - Rat brain using NF-L Rabbit mAb (NBP3-15874) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NF-L-Antibody-3L9Y10-Immunohistochemistry-Paraffin-NBP3-15874-img0002.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (3L9Y10) [NBP3-15874] - Rat brain using NF-L Rabbit mAb (NBP3-15874) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Immunohistochemistry: NF-L Antibody (3L9Y10) [NBP3-15874] - Immunofluorescence analysis of rat brain using NF-L Rabbit mAb (NBP3-15874) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-3L9Y10-Immunohistochemistry-NBP3-15874-img0003.jpg "Immunohistochemistry: NF-L Antibody (3L9Y10) [NBP3-15874] - Immunofluorescence analysis of rat brain using NF-L Rabbit mAb (NBP3-15874) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunoprecipitation: NF-L Antibody (3L9Y10) [NBP3-15874] - Immunoprecipitation analysis of 300ug extracts of SH-SY5Y cells using 3ug NF-L antibody (NBP3-15874). Western blot was performed from the immunoprecipitate using NF-L antibody (NBP3-15874) at a dilition of 1:10000.](http://images.novusbio.com/fullsize/NF-L-Antibody-3L9Y10-Immunoprecipitation-NBP3-15874-img0007.jpg "Immunoprecipitation: NF-L Antibody (3L9Y10) [NBP3-15874] - Immunoprecipitation analysis of 300ug extracts of SH-SY5Y cells using 3ug NF-L antibody (NBP3-15874). Western blot was performed from the immunoprecipitate using NF-L antibody (NBP3-15874) at a dilition of 1:10000.")
![Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517235788.jpg "Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517152736.jpg "Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of paraffin-embedded Rat brain tissue using NF-L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517172848.jpg "Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of paraffin-embedded Rat brain tissue using NF-L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.")
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of Human brain tissue using NF-L Rabbit mAb . DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517162336.jpg "Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of Human brain tissue using NF-L Rabbit mAb . DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.")
![Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-5220251721355.jpg "Immunohistochemistry: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using NF-L Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of paraffin-embedded Mouse brain tissue using NF-L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517184520.jpg "Immunocytochemistry/ Immunofluorescence: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Confocal imaging of paraffin-embedded Mouse brain tissue using NF-L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.")
![Western Blot: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Western blot analysis of lysates from SH-SY5Y cells using NF-L Rabbit mAb at 1:100000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.](http://images.novusbio.com/fullsize/nbp3-15874_rabbit-nf-l-mab-3l9y10-52202517235717.jpg "Western Blot: NF-L Antibody (3L9Y10) - Azide and BSA Free [NF-L] - Western blot analysis of lysates from SH-SY5Y cells using NF-L Rabbit mAb at 1:100000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.")
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
![Immunohistochemistry Desmin Antibody [Unconjugated]](https://images.novusbio.com/images2/Desmin_AF3844_Immunohistochemistry_10689.jpg)
![Immunohistochemistry Desmin Antibody [Unconjugated]](https://images.novusbio.com/images2/Desmin_AF3844_Immunohistochemistry_6808.jpg)
![Immunocytochemistry/ Immunofluorescence Desmin Antibody [Unconjugated]](https://images.novusbio.com/images/af3844_human-mouse-desmin-affinity-purified-polyclonal-ab-41202410331920.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")