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Neuroplastin 65 Antibody [Unconjugated]

 (11 Publications)
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Neuroplastin 65 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 3 µg/mL Goat Anti-Human/Mouse Neuroplastin 65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5360) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neuroplastin 65 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 3 µg/mL Goat Anti-Human/Mouse Neuroplastin 65 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Western blot shows lysates of human brain and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Neuroplastin 65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5360) followed ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF5360-SP
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Neuroplastin 65 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neuroplastin 65
Gln29-His336
Accession # NP_036560
Specificity
Detects human and mouse Neuroplastin 65 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Neuroplastin 55 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NPTN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5360 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuroplastin 65 Antibody [Unconjugated]

  • GP55;GP65;np55;np65;NPTN;SDFR1;SDR1
  • Neuroplastin 65
  • Np65

Background

Neuroplastin (NPTN; also stromal cell-derived receptor 1 and Np55) is a 52-57 kDa member of the Ig-superfamily. It is widely expressed and likely serves as a cell adhesion molecule in multiple tissues. Human Neuroplastin is 282 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains two Ig-like domains (aa 32-119 and 122-213) and a 38 aa cytoplasmic region (aa 245-282). There is a brain-enriched 65 kDa form (Np65) that negatively regulates LTP in the hippocampus. It contains an N terminal 116 aa Ig-like domain-containing insert after Asn30 and interacts homotypically. Notably, human and rodent Np65 expression patterns are different in brain, with rodent Np65 not predominating in cerebellum. Over aa 29-336, human Np65 is 95% aa identical to mouse Np65.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neuroplastin 65 Antibody (AF5360)(11)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 4 applications: ICC, IHC-Fr, Immunohistochemistry, Western Blot.


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ICC
(1)
IHC-Fr
(1)
Immunohistochemistry
(1)
Western Blot
(2)
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Mouse
(3)
Rat
(1)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF5360 Applications Species
Cheng, J;Chen, L;Zheng, YN;Liu, J;Zhang, L;Zhang, XM;Huang, L;Yuan, QL; Disfunction of dorsal raphe nucleus-hippocampus serotonergic-HTR3 transmission results in anxiety phenotype of Neuroplastin 65-deficient mice Acta pharmacologica Sinica 2024-03-25 [PMID: 38528118] (Immunohistochemistry, Western Blot, Mouse, Transgenic Mouse) Immunohistochemistry, Western Blot Mouse, Transgenic Mouse
Wu, DD;Cheng, J;Zheng, YN;Liu, YT;Hou, SX;Liu, LF;Huang, L;Yuan, QL; Neuroplastin 65 deficiency reduces amyloid plaque formation and cognitive deficits in an Alzheimer's disease mouse model Frontiers in cellular neuroscience 2023-05-05 [PMID: 37213217] (Western Blot, Mouse) Western Blot Mouse
Kershberg L, Banerjee A, Kaeser PS. Protein composition of axonal dopamine release sites in the striatum eLife 2022-12-29 [PMID: 36579890]
William R. Goodyer, Benjamin M. Beyersdorf, Lauren Duan, Nynke S. van den Berg, Sruthi Mantri, Francisco X. Galdos et al. In vivo visualization and molecular targeting of the cardiac conduction system Journal of Clinical Investigation 2022-10-17 [PMID: 35951416]
Sherylanne Newton, Fanbo Kong, Adam J. Carlton, Carlos Aguilar, Andrew Parker, Gemma F. Codner et al. Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing PLOS Genetics 2022-01-31 [PMID: 35100259]
Ilic K, Lin X, Malci A et al. Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts International Journal of Molecular Sciences 2021-12-18 [PMID: 34948386]
Marta Balog, Senka Blažetić, Vedrana Ivić, Irena Labak, Bartosz Krajnik, Raquel Marin et al. Disarranged neuroplastin environment upon aging and chronic stress recovery in female Sprague Dawley rats European Journal of Neuroscience 2022-05-01 [PMID: 33909305]
Katarina Ilic, Kristina Mlinac-Jerkovic, Natasa Jovanov-Milosevic, Goran Simic, Nikola Habek, Nenad Bogdanovic et al. Hippocampal expression of cell-adhesion glycoprotein neuroplastin is altered in Alzheimer's disease Journal of Cellular and Molecular Medicine 2019-02-01 [PMID: 30488668]
Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function J Neurosci, 2016-08-31;36(35):9201-16. 2016-08-31 [PMID: 27581460] (IHC-Fr, Mouse) IHC-Fr Mouse
Leanne Carrott, Michael R. Bowl, Carlos Aguilar, Stuart L. Johnson, Lauren Chessum, Melissa West et al. Absence of Neuroplastin-65 Affects Synaptogenesis in Mouse Inner Hair Cells and Causes Profound Hearing Loss The Journal of Neuroscience 2016-01-06 [PMID: 26740663]
Show All 11 Publications.

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Bioinformatics

Gene Symbol NPTN
Uniprot