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Rat NCAM-1/CD56 ELISA Kit (Colorimetric)

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ELISA: Rat NCAM-1/CD56 ELISA Kit (Colorimetric) [NBP2-81181] - Samples were spiked with high concentrations of Rat NCAM-1/CD56 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat NCAM-1/CD56 ELISA Kit (Colorimetric) [NBP2-81181] - Samples were spiked with high concentrations of Rat NCAM-1/CD56 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Rat NCAM-1/CD56 ELISA Kit (Colorimetric) [NBP2-81181] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL

Order Details

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Rat NCAM-1/CD56 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat NCAM-1/CD56 in samples. No significant cross-reactivity or interference between Rat NCAM-1/CD56 and analogues was observed.
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.18%
Intra-Assay
CV% < 5.43%
Spike Recovery
88-107%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
NCAM1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat NCAM-1/CD56 ELISA Kit (Colorimetric)

  • CD56 / NCAM-1
  • CD56 antigen
  • CD56
  • MSK39
  • NCAM1
  • N-CAM-1
  • NCAM-1
  • NCAMantigen recognized by monoclonal 5.1H11
  • neural cell adhesion molecule 1
  • neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Rat NCAM-1/CD56 ELISA Kit (Colorimetric) Manual
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Bioinformatics

Gene Symbol NCAM1