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NCAM-1/CD56 Antibody (301021) [Unconjugated]

 (9 Publications)
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Western blot shows lysates of human brain (cerebellum and hypothalamus). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB24081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NCAM‑1/CD56 at approximately 105-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of human brain (cerebellum and hypothalamus). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB24081) followed by HRP-conjugated ...read more
Human PBMCs were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody ...read more
Human NK cells were expanded from PBMCs using Cloudz Human NK Cell Expansion Kit (CLD004) and were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype ...read more
NCAM-1/CD56 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) at 25 µg/mL over-night at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
301021
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB24081U-100UG
Alexa Fluor 405
FAB24081V-100UG
Alexa Fluor 488
FAB24081G-100UG
Alexa Fluor 594
FAB24081T-100UG
Alexa Fluor 647
FAB24081R-100UG
Alexa Fluor 700
FAB24081N-100UG
Alexa Fluor 750
FAB24081S-100UG
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB24081-SP
Carrier Free
FAB24081V-100UG
Carrier Free
FAB24081U-100UG
Carrier Free
FAB24081T-100UG
Carrier Free
FAB24081S-100UG
Carrier Free
FAB24081R-100UG
Carrier Free
FAB24081N-100UG
Carrier Free
FAB24081G-100UG
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NCAM-1/CD56 Antibody (301021) [Unconjugated] Summary

Specificity
Detects human NCAM-1/CD56 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM-1-L1, recombinant mouse (rm) MAdCAM-1, rmNCAM-1-L1, or rmOCAM is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NCAM1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB24081 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NCAM-1/CD56 Antibody (301021) [Unconjugated]

  • CD56 / NCAM-1
  • CD56 antigen
  • CD56
  • MSK39
  • NCAM1
  • N-CAM-1
  • NCAM-1
  • NCAMantigen recognized by monoclonal 5.1H11
  • neural cell adhesion molecule 1
  • neural cell adhesion molecule, NCAM

Background

Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4‑6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7‑8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10‑13).

  1. Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49.
  2. Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.
  3. Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.
  4. Dickson, G. et al. (1987) Cell 50:1119.
  5. Lanier, L.L. et al. (1991) J. Immunol. 146:4421.
  6. Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.
  7. Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.
  8. Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.
  9. Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.
  10. Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.
  11. Doherty, P. et al. (1995) J. Neurobiol. 26:437.
  12. Eckardt, M. et al. (2000) J. Neurosci. 20:5234.
  13. Muller, D. et al. (1996) Neuron 17:413.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NCAM-1/CD56
Antibody (301021) [Un ...
MAB24081
NCAM-1/CD56 Antibody (301021) [Unconjugated]
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready

Publications for NCAM-1/CD56 Antibody (MAB24081)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC-Fr, IHC-P.


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(4)
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Showing Publications 1 - 9 of 9.
Publications using MAB24081 Applications Species
Aouimeur, I;Sagnial, T;Coulomb, L;Maurin, C;Thomas, J;Forestier, P;Ninotta, S;Perrache, C;Forest, F;Gain, P;Thuret, G;He, Z; Investigating the Role of TGF-? Signaling Pathways in Human Corneal Endothelial Cell Primary Culture Cells 2023-06-14 [PMID: 37371094] (ICC, Human) ICC Human
Eun Ah Ye, Ho Seok Chung, Yoonkyung Park, Jeong Hye Sunwoo, Whanseo Lee, Jin Kim et al. Induction of Corneal Endothelial-like Cells from Mesenchymal Stem Cells of the Umbilical Cord International Journal of Molecular Sciences 12/6/2022 [PMID: 36499735]
Guoxu Zheng, Zhangyan Guo, Weimiao Li, Wenjin Xi, Baile Zuo, Rui Zhang et al. Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab Signal Transduction and Targeted Therapy 6/23/2021 [PMID: 34158475]
X Wang, M Lu, X Tian, Y Ren, Y Li, M Xiang, S Chen Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkey Stem Cell Res Ther, 2020-08-03;11(1):334. 2020-08-03 [PMID: 32746912] (Human) Human
Sylvie Berthier, Louis Larrouquère, Pierre Champelovier, Edwige Col, Christine Lefebvre, Cécile Cottet-Rouselle et al. A New Patient-Derived Metastatic Glioblastoma Cell Line: Characterisation and Response to Sodium Selenite Anticancer Agent Cancers (Basel) 12/21/2018 [PMID: 30583471]
T Frenzel, B Hoffmann, R Schmitz, A Bethge, U Schumacher, G Wedemann Radiotherapy and chemotherapy change vessel tree geometry and metastatic spread in a small cell lung cancer xenograft mouse tumor model PLoS ONE, 2017-11-06;12(11):e0187144. 2017-11-06 [PMID: 29107953] (IHC-P, Mouse) IHC-P Mouse
Zhiguo He, Fabien Forest, Philippe Gain, Damien Rageade, Aurélien Bernard, Sophie Acquart et al. 3D map of the human corneal endothelial cell Scientific Reports 7/6/2016 [PMID: 27381832]
Heidemann F, Schildt A, Schmid K, Bruns O, Riecken K, Jung C, Ittrich H, Wicklein D, Reimer R, Fehse B, Heeren J, Luers G, Schumacher U, Heine M Selectins mediate small cell lung cancer systemic metastasis. PLoS ONE, 2014-04-03;9(4):e92327. 2014-04-03 [PMID: 24699516] (Flow Cytometry, Human) Flow Cytometry Human
Theriault ME, Pare MÈ, Maltais F, Debigare R Satellite cells senescence in limb muscle of severe patients with COPD. PLoS ONE, 2012-06-13;7(6):e39124. 2012-06-13 [PMID: 22720047] (IHC-Fr, Human) IHC-Fr Human

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Bioinformatics

Gene Symbol NCAM1
Uniprot