As part of an Endometriosis-related project in horses, I would like to ask some information with regard to this. I would like to know about its tested or predicted reactivity with horse.

Name: Nanog Antibody
Brand: Novus Biologicals
SKU: NB110-40660
Price: 399 USD

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Accepted Answer

This antibody has not yet been tested with horse. This means that use of this antibody with horse would not be covered by our guarantee; however, you would be eligible for our Innovator's Reward Program. For an indication of the likely cross-reactivity (how well the antibody is likely to perform with horse), I have run UniProt Blast sequence comparison of the immunogen sequence versus horse proteins. For Nanog Antibody NB110-40660, its immunogen is shown on its web page datasheet. The result of the UniProt Blast was a 93% match to both F6PPY5 and F6PXN9. 93% means high homology, and suggests that this antibody would be suitable for horse samples, although we cannot guarantee it. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Po, CaSpecies Glossary
Applications	WB, ELISA, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	0.5 mg/ml

Immunogen	Peptide with sequence C-QNQRMKSKRWQKNN corresponding to internal region according to NP_079141.2.
Marker	Embryonic Stem Cell Marker
Predicted Species	Canine (100%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	NANOG
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative	0.02% Sodium Azide
Concentration	0.5 mg/ml
Purity	Immunogen affinity purified

Publications using NB110-40660	Applications	Species
Aschacher T, Schmidt K, Aschacher O et al. Telocytes in the human ascending aorta: Characterization and exosome-related KLF-4/VEGF-A expression Journal of cellular and molecular medicine 2021-09-25 [PMID: 34562312] (ICC/IF, Human)	ICC/IF	Human
Vassena R, Montserrat N, Carrasco Canal B et al. Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells. Hum Mol Genet 2012-08-01 [PMID: 22547223] (IHC-P, Human)	IHC-P	Human
Vackova I, Novakova Z, Krylov V et al. Analysis of marker expression in porcine cell lines derived from blastocysts produced in vitro and in vivo J Reprod Dev 2011-10-01 [PMID: 21685711] (ICC/IF, Porcine)	ICC/IF	Porcine
Salisbury E, Rodenberg E, Sonnet C et al. Sensory nerve induced inflammation contributes to heterotopic ossification. J Cell Biochem. 2011-10-01 [PMID: 21678472] (IHC-P, Mouse)	IHC-P	Mouse
Monaco-Shawver L, Schwartz L, Tuluc F et al. Substance P inhibits natural killer cell cytotoxicity through the neurokinin-1 receptor. J Leukoc Biol;89(1):113-25. 2011-01-01 [PMID: 20940324]
Raya A, Rodriguez-Piza I, Guenechea G et al. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature 2009-07-02 [PMID: 19483674]
Aasen T. Efficient rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol 26(11):1276-84. 2008-11-01 [PMID: 18931654]
Hyslop L, Stojkovic M, Armstrong L et al. Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages. Stem Cells 2005-09-01 [PMID: 15983365]

Nanog Antibody

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Gene Symbol	NANOG
Entrez	79923(Human) 71950(Mouse) 414065(Rat)
Uniprot	Q9H9S0(Human)