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n-Myc Antibody (NMYC-1) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Flow, GS, ICC/IF, IP, ChIP, CyTOF-ready
Clone
NMYC-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594

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n-Myc Antibody (NMYC-1) [Alexa Fluor® 594] Summary

Immunogen
A synthetic peptide (SPYVESEDAPPQKC) conjugated to KLH, corresponding to amino acids 327-339 of human n-Myc. [UniProt# P04198]
Localization
Nuclear, some cytoplasmic.
Specificity
This antibody does not react with c-Myc or L-Myc.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYCN
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation
  • CyTOF-ready
  • Flow Cytometry
  • Gel Super Shift Assays
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for n-Myc Antibody (NMYC-1) [Alexa Fluor® 594]

  • BHLHE37
  • bHLHe37N-myc proto-oncogene protein
  • Class E basic helix-loop-helix protein 37
  • MODED
  • neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene
  • N-myc
  • NMYCneuroblastoma MYC oncogene
  • ODED
  • oncogene NMYC
  • pp65/67
  • v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
  • v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)

Background

n-Myc is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for n-Myc Antibody (NB200-109AF594) (0)

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Secondary Antibodies

 

Isotype Controls

Additional n-Myc Products

Research Areas for n-Myc Antibody (NB200-109AF594)

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Blogs on n-Myc.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

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Bioinformatics

Gene Symbol MYCN