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Myosin heavy chain 14 Antibody

Images

 
Western Blot: Myosin Heavy Chain Antibody [NBP2-49533] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: Myosin Heavy Chain Antibody [NBP2-49533] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Myosin Heavy Chain Antibody [NBP2-49533] - Analysis in human small intestine and lymph node tissues. Corresponding MYH14 RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: Myosin Heavy Chain Antibody [NBP2-49533] - Staining of human lymph node shows no cytoplasmic positivity in germinal center cells as expected.
Immunohistochemistry-Paraffin: Myosin Heavy Chain Antibody [NBP2-49533] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Myosin heavy chain 14 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: FTTRTVRQVFRLEEGVASDEEAEEAQPGSGPSPEPEGSPP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYH14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Myosin Heavy Chain Recombinant Protein Antigen (NBP2-49533PEP)

Reactivity Notes

Mouse (80%), Rat (80%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Myosin heavy chain 14 Antibody

  • DFNA4
  • DFNA4A
  • FP17425
  • MHC16
  • MYH14
  • MYH17
  • myosin, heavy chain 14, non-muscle
  • NMHC II-C
  • NMHC-II-C
  • PNMHH

Background

The MYH14 gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYH14