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Human Myelin PLP ELISA Kit (Colorimetric)

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ELISA: Human Myelin PLP ELISA Kit (Colorimetric) [NBP3-00412] - Samples were spiked with high concentrations of Human Myelin PLP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Myelin PLP ELISA Kit (Colorimetric) [NBP3-00412] - Samples were spiked with high concentrations of Human Myelin PLP and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Human Myelin PLP ELISA Kit (Colorimetric) [NBP3-00412] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

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Human Myelin PLP ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Myelin PLP in samples. No significant cross-reactivity or interference between Human Myelin PLP and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.13%
Intra-Assay
4.85%
Spike Recovery
87 - 110%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
PLP1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Myelin PLP ELISA Kit (Colorimetric)

  • DM-20
  • HLD1
  • Lipophilin
  • major myelin proteolipid protein
  • MMPL
  • Myelin PLP
  • myelin proteolipid protein
  • PLP
  • PLP/DM20
  • PLP1
  • PMD
  • proteolipid protein 1
  • spastic paraplegia 2, uncomplicated
  • SPG2

Background

Human PLP (also known as lipophilin) is a 30 kDa protein (277 amino acids) found in myelin of the CNS and PNS. Expressed by oligodendrocytes and Schwann cells, PLP stabilizes myelin by preventing lipid bilayer fusion, and aids in compaction. Different mutations of the human PLP gene product result in two neurological disorders - Pelizaeus-Merzbacher disease and Spastic Paraplegia type 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Human Myelin PLP ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol PLP1