![Western Blot: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965] - Western Blot Analysis of human HeLa, A431, HepG2, HAP1, U937 cell lysates using MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).](http://images.novusbio.com/fullsize/MTAP-Antibody-MTAP-3137R-Azide-and-BSA-Free-Western-Blot-NBP3-08965-img0003.jpg "Western Blot: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965] - Western Blot Analysis of human HeLa, A431, HepG2, HAP1, U937 cell lysates using MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | MTAP/3137R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07220). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary) (Uniprot: Q13126) |
| Localization | Cytoplasmic |
| Marker | Tumor Suppressor Marker |
| Specificity | Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MTAP |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: Use antibody at 2-4ug/ml. For coating, order antibody without BSA |
| Theoretical MW | 31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MTAP |
![Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).](http://images.novusbio.com/fullsize/MTAP-Antibody-MTAP-3137R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08965-img0002.jpg "Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).")
![Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).](http://images.novusbio.com/fullsize/MTAP-Antibody-MTAP-3137R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08965-img0001.jpg "Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) - Azide and BSA Free [NBP3-08965] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")