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![Western Blot: MTAP Antibody (2G4) [H00004507-M01] - MTAP monoclonal antibody (M01), clone 2G4. Analysis of MTAP expression in NIH/3T3.](http://images.novusbio.com/fullsize/MTAP-Antibody-2G4-Western-Blot-H00004507-M01-img0013.jpg "Western Blot: MTAP Antibody (2G4) [H00004507-M01] - MTAP monoclonal antibody (M01), clone 2G4. Analysis of MTAP expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: MTAP Antibody (2G4) [H00004507-M01] - Analysis of monoclonal antibody to MTAP on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MTAP-Antibody-2G4-Immunocytochemistry-Immunofluorescence-H00004507-M01-img0008.jpg)
![Western Blot: MTAP Antibody (2G4) [H00004507-M01] - MTAP monoclonal antibody (M01), clone 2G4 Analysis of MTAP expression in HeLa.](http://images.novusbio.com/fullsize/MTAP-Antibody-2G4-Western-Blot-H00004507-M01-img0010.jpg)
![Western Blot: MTAP Antibody (2G4) [H00004507-M01] - MTAP monoclonal antibody (M01), clone 2G4. Analysis of MTAP expression in PC-12.](http://images.novusbio.com/fullsize/MTAP-Antibody-2G4-Western-Blot-H00004507-M01-img0011.jpg)
![Western Blot: MTAP Antibody (2G4) [H00004507-M01] - MTAP monoclonal antibody (M01), clone 2G4. Analysis of MTAP expression in Raw 264.7.](http://images.novusbio.com/fullsize/MTAP-Antibody-2G4-Western-Blot-H00004507-M01-img0012.jpg)
![ELISA: MTAP Antibody (2G4) [H00004507-M01] - Detection limit for recombinant GST tagged MTAP is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MTAP-Antibody-2G4-ELISA-H00004507-M01-img0014.jpg)
![Western Blot: MTAP Antibody (JE63-74) [NBP3-32610] - Western blot analysis of MTAP on different lysates with Rabbit anti-MTAP antibody (NBP3-32610) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32610) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32610_rabbit-mtap-mab-je63-74-278202415295529.jpg "Western Blot: MTAP Antibody (JE63-74) [NBP3-32610] - Western blot analysis of MTAP on different lysates with Rabbit anti-MTAP antibody (NBP3-32610) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32610) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MTAP Antibody (JE63-74) [NBP3-32610] - Immunofluorescence analysis of paraffin-embedded mouse liver tissue labeling MTAP with Rabbit anti-MTAP antibody (NBP3-32610) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32610, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32610_rabbit-mtap-mab-je63-74-298202414333674.jpg)
![Immunohistochemistry: MTAP Antibody (JE63-74) [NBP3-32610] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-MTAP antibody (NBP3-32610) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32610) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32610_rabbit-mtap-mab-je63-74-298202413585517.jpg)
![Immunocytochemistry/ Immunofluorescence: MTAP Antibody (JE63-74) [NBP3-32610] - Immunocytochemistry analysis of NIH/3T3 cells labeling MTAP with Rabbit anti-MTAP antibody (NBP3-32610) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MTAP antibody (NBP3-32610) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32610_rabbit-mtap-mab-je63-74-29202410484843.jpg)
![Flow Cytometry: MTAP Antibody (JE63-74) [NBP3-32610] - Flow cytometric analysis of NIH/3T3 cells labeling MTAP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32610, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32610_rabbit-mtap-mab-je63-74-392024884673.jpg)
![Western Blot: MTAP Antibody [NBP1-32718] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MTAP antibody diluted at a dilution of 1:5000.](http://images.novusbio.com/fullsize/MTAP-Antibody-Western-Blot-NBP1-32718-img0009.jpg "Western Blot: MTAP Antibody [NBP1-32718] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MTAP antibody diluted at a dilution of 1:5000.")
![Immunocytochemistry/Immunofluorescence: MTAP Antibody [NBP1-32718] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTAP protein stained by MTAP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/MTAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32718-img0007.jpg)
![Immunohistochemistry-Paraffin: MTAP Antibody [NBP1-32718] - SAS xenograft , using MTAP antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MTAP-Antibody-Immunohistochemistry-Paraffin-NBP1-32718-img0003.jpg)
![Western Blot: MTAP Antibody [NBP1-32718] - Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MTAP-Antibody-Western-Blot-NBP1-32718-img0004.jpg)
![Immunoprecipitation: MTAP Antibody [NBP1-32718] - Immunoprecipitation of MTAP protein from HeLa whole cell extracts using 5 ug of MTAP antibody [N1C3]. Western blot analysis was performed using MTAP antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MTAP-Antibody-Immunoprecipitation-NBP1-32718-img0008.jpg)
![Western Blot: MTAP Antibody (MTAP/1813) [NBP2-75730] - Western Blot Analysis of Human HeLa, A431, HePG2 and HAP1 cell lysate using MTAP Antibody (MTAP/1813).](http://images.novusbio.com/fullsize/MTAP-Antibody-MTAP-1813-Western-Blot-NBP2-75730-img0005.jpg "Western Blot: MTAP Antibody (MTAP/1813) [NBP2-75730] - Western Blot Analysis of Human HeLa, A431, HePG2 and HAP1 cell lysate using MTAP Antibody (MTAP/1813).")
![Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/1813) [NBP2-75730] - Formalin-fixed, paraffin-embedded Human Kidney stained with MTAP Mouse Monoclonal Antibody (MTAP/1813).](http://images.novusbio.com/fullsize/MTAP-Antibody-MTAP-1813-Immunohistochemistry-Paraffin-NBP2-75730-img0001.jpg)
![Protein Array: MTAP Antibody (MTAP/1813) [NBP2-75730] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MTAP Antibody (MTAP/1813). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/MTAP-Antibody-MTAP-1813-Protein-Array-NBP2-75730-img0004.jpg)
![Western Blot: MTAP Antibody (MTAP/3137R) [NBP3-07220] - Western Blot Analysis of human HeLa, A431, HepG2, HAP1, U937 cell lysates using MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).](http://images.novusbio.com/fullsize/MTAP-Antibody-MTAP-3137R-Western-Blot-NBP3-07220-img0003.jpg "Western Blot: MTAP Antibody (MTAP/3137R) [NBP3-07220] - Western Blot Analysis of human HeLa, A431, HepG2, HAP1, U937 cell lysates using MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).")
![Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) [NBP3-07220] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).](http://images.novusbio.com/fullsize/MTAP-Antibody-MTAP-3137R-Immunohistochemistry-Paraffin-NBP3-07220-img0002.jpg)
![Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/3137R) [NBP3-07220] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Rabbit Monoclonal Antibody (MTAP/3137R).](http://images.novusbio.com/fullsize/MTAP-Antibody-MTAP-3137R-Immunohistochemistry-Paraffin-NBP3-07220-img0001.jpg)
![Western Blot: MTAP Antibody [NBP2-56796] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/MTAP-Antibody-Western-Blot-NBP2-56796-img0002.jpg "Western Blot: MTAP Antibody [NBP2-56796] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: MTAP Antibody [NBP2-56796] - Staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/MTAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56796-img0001.jpg)
![Western Blot: MTAP Antibody (5A6V10) [NBP3-33318] - Western blot analysis of lysates from Rat liver, using MTAP Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp3-33318_rabbit-mtap-mab-5a6v10-1012202411334086.jpg "Western Blot: MTAP Antibody (5A6V10) [NBP3-33318] - Western blot analysis of lysates from Rat liver, using MTAP Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 120s.")
![Western Blot: MTAP Antibody (5A6V10) [NBP3-33318] - Western blot analysis of various lysates using MTAP Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33318_rabbit-mtap-mab-5a6v10-1012202411381216.jpg)
![Western Blot: MTAP Antibody (S07-5A0) [NBP3-14956] - Western blot detection of MTAP in A549, MCF-7, U251, HL-60 cell lysates using MTAP Rabbit mAb (1:1000 diluted). Predicted band size: 31kDa. Observed band size: 31kDa.](http://images.novusbio.com/fullsize/MTAP-Antibody-S07-5A0-Western-Blot-NBP3-14956-img0001.jpg "Western Blot: MTAP Antibody (S07-5A0) [NBP3-14956] - Western blot detection of MTAP in A549, MCF-7, U251, HL-60 cell lysates using MTAP Rabbit mAb (1:1000 diluted). Predicted band size: 31kDa. Observed band size: 31kDa.")
![Immunohistochemistry-Paraffin: MTAP Antibody (rMTAP/1813) - Azide and BSA Free [NBP3-08964] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).](http://images.novusbio.com/fullsize/MTAP-Antibody-rMTAP-1813-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08964-img0001.jpg "Immunohistochemistry-Paraffin: MTAP Antibody (rMTAP/1813) - Azide and BSA Free [NBP3-08964] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).")
![Immunohistochemistry-Paraffin: MTAP Antibody (rMTAP/1813) - Azide and BSA Free [NBP3-08964] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).](http://images.novusbio.com/fullsize/MTAP-Antibody-rMTAP-1813-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08964-img0002.jpg)
![Immunohistochemistry-Paraffin: MTAP Antibody (rMTAP/1813) [NBP3-07219] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).](http://images.novusbio.com/fullsize/MTAP-Antibody-rMTAP-1813-Immunohistochemistry-Paraffin-NBP3-07219-img0002.jpg "Immunohistochemistry-Paraffin: MTAP Antibody (rMTAP/1813) [NBP3-07219] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).")
![Immunohistochemistry-Paraffin: MTAP Antibody (rMTAP/1813) [NBP3-07219] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).](http://images.novusbio.com/fullsize/MTAP-Antibody-rMTAP-1813-Immunohistochemistry-Paraffin-NBP3-07219-img0001.jpg)
![Western Blot: MTAP Overexpression Lysate (Adult Normal) [NBP2-08113] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MTAP.](http://images.novusbio.com/fullsize/MTAP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08113-img0001.jpg "Western Blot: MTAP Overexpression Lysate (Adult Normal) [NBP2-08113] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MTAP.")
![Sandwich ELISA: MTAP Antibody (4C8) [H00004507-M08] - Detection limit for recombinant GST tagged MTAP is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MTAP-Antibody-4C8-Sandwich-ELISA-H00004507-M08-img0001.jpg "Sandwich ELISA: MTAP Antibody (4C8) [H00004507-M08] - Detection limit for recombinant GST tagged MTAP is 0.3 ng/ml as a capture antibody.")