MTAP Antibody (rMTAP/1813)

Recombinant Monoclonal Antibody

Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary) (Uniprot: Q13126)

Cytoplasmic

Tumor Suppressor Marker

Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.

IgG1 Kappa

Monoclonal

Mouse

MTAP

Protein A or G purified

Test in a species/application not listed above to receive a full credit towards a future purchase.

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.