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MST2/STK3 Antibody

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Western Blot: MST2/STK3 Antibody [NBP2-19421] - STK3 antibody detects STK3 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STK3 ...read more
Western Blot: MST2 Antibody [NBP2-19421] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: STK3 transfected 293T lysates 7. 5% SDS PAGE gel, diluted at 1:5000.
Western Blot: MST2 Antibody [NBP2-19421] - Sample (30 ug of whole cell lysate) A: THP-1 B: NCI-H929 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: MST2/STK3 Antibody [NBP2-19421] - STK3 antibody immunoprecipitates STK3 protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MST2/STK3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MST2/STK3. The exact sequence is proprietary.
Localization
Cytoplasm (By similarity); Nucleus (By similarity)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STK3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:10000
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for MST2/STK3 Antibody

  • EC 2.7.11
  • FLJ90748
  • KRS1
  • Mammalian STE20-like protein kinase 2
  • Mess1
  • MST2
  • MST-2
  • MST2EC 2.7.11.1
  • MST3
  • serine/threonine kinase 3 (STE20 homolog, yeast)
  • serine/threonine kinase 3 (Ste20, yeast homolog)
  • serine/threonine kinase 3
  • serine/threonine-protein kinase 3
  • Serine/threonine-protein kinase Krs-1
  • STE20-like kinase MST2
  • STK3

Background

Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol STK3
Uniprot