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Monoamine Oxidase B Antibody

Images

 
Orthogonal Strategies: Western Blot: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody ...read more
Immunocytochemistry/ Immunofluorescence: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted ...read more
Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse liver. Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 ...read more
Western Blot: Monoamine Oxidase B Antibody [NBP1-32426] - Sample (50 ug of whole cell lysate) A: Mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse lung. Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
ELISA: Monoamine Oxidase B Antibody [NBP1-32426] - ELISA detection of MAOB using Monoamine Oxidase B antibody [N2C3] for capture at a concentration of 2.5 ug/mL and monoamine oxidase B for detection at a concentration ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Monoamine Oxidase B Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Monoamine Oxidase B. The exact sequence is proprietary.
Localization
Mitochondrion outer membrane
Marker
Mitochondrial Outer Membrane Marker
Predicted Species
Porcine (90%), Bovine (90%), Canine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAOB
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:1000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Sandwich ELISA Assay dependent
  • Western Blot 1:500-1:3000
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%), Guinea Pig (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Monoamine Oxidase B Antibody

  • adrenalin oxidase
  • amine oxidase [flavin-containing] B
  • EC 1.4.3
  • EC 1.4.3.21
  • EC 1.4.3.4
  • MAO, brain
  • MAO, platelet
  • MAOB
  • MAO-B
  • MGC26382
  • Monoamine Oxidase B
  • Monoamine oxidase type B
  • tyramine oxidase

Background

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAOB