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VAP-1/AOC3 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NBP1-89671] - Analysis in human lung and skeletal muscle tissues. Corresponding AOC3 RNA-seq data are presented for the same tissues.
Western Blot: VAP-1/AOC3 Antibody [NBP1-89671] - Analysis in control (vector only transfected HEK293T lysate) and aOC3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NBP1-89671] - Equine endometrium. All vascular smooth muscle was positively stained. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NBP1-89671] - Staining of human lung shows high expression.
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NBP1-89671] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NBP1-89671] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NBP1-89671] - Staining of human skin shows no positivity in squamous epithelial cells as expected.

Product Details

Summary
Reactivity Hu, EqSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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VAP-1/AOC3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DIDQMIFNRELPQASGLLHHCCFYKHRGRNLVTMTTAPRGLQSGDRATWFGLYYNISGAGFFLHHVGLELLVNHKALDPARWTIQKVFYQGRYYDSLAQLEAQFEAGLVNVVLI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AOC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
VAP-1/AOC3 Protein (NBP1-89671PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-89671 in the following applications:

Publications
Read Publications using
NBP1-89671 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (82%). Human reactivity reported in scientific literature (PMID: 25562318). Equine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VAP-1/AOC3 Antibody

  • amine oxidase, copper containing 3 (vascular adhesion protein 1)
  • AOC3
  • Copper amine oxidase
  • EC 1.4.3
  • HPAO
  • HPAOSSAO
  • Semicarbazide-sensitive amine oxidase
  • SSAO
  • VAP1
  • VAP-1
  • VAP1EC 1.4.3.21
  • VAP-1membrane primary amine oxidase
  • Vascular adhesion protein 1

Background

Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity. A pseudogene for this gene has been described and is located approximately 9-kb downstream.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAP-1/AOC3 Antibody (NBP1-89671)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Human
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Review for VAP-1/AOC3 Antibody (NBP1-89671) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Equine.

Reviews using NBP1-89671:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Equine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin VAP-1/AOC3 NBP1-89671
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5
reviewed by:
Verified Customer
IHC-P Equine 02/20/2020
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedEndometrium
SpeciesEquine
LotC117560

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for VAP-1/AOC3 Antibody (NBP1-89671) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for VAP-1/AOC3 Antibody (NBP1-89671)

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Blogs on VAP-1/AOC3

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
02/20/2020
Application: IHC-P
Species: Equine

Bioinformatics

Gene Symbol AOC3