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Monoamine Oxidase B Antibody

Images

 
Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Monoamine Oxidase B Antibody [NB100-2826] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) ...read more
Flow Cytometry: Monoamine Oxidase B Antibody [NB100-2826] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa ...read more
Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Staining of Human Liver (A) with antibody at 0.1 ug/mL and Testes (B) and Breast Cancer (C) lysate with antibody at 0.3ug/mL (35 ug protein in RIPA buffer). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Monoamine Oxidase B Antibody Summary

Immunogen
Peptide with sequence C-HKARKLARLTKEE corresponding to internal region according to NP_000889.3.
Specificity
This antibody is not expected to cross-react with MAOA.
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAOB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx 60 kDa band observed in Human and Mouse Liver lysates and approx. 55-60 kDa in Human Testis and Breast cancer lysates (calculated MW of 58.8 kDa according to NP_000889.3).
Control
Monoamine Oxidase B Overexpression Lysate
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-2826.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Monoamine Oxidase B Antibody

  • adrenalin oxidase
  • amine oxidase [flavin-containing] B
  • EC 1.4.3
  • EC 1.4.3.21
  • EC 1.4.3.4
  • MAO, brain
  • MAO, platelet
  • MAOB
  • MAO-B
  • MGC26382
  • Monoamine Oxidase B
  • Monoamine oxidase type B
  • tyramine oxidase

Background

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Monoamine Oxidase B Antibody (NB100-2826)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MAOB
Entrez
Uniprot