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MLL5 Antibody - Azide and BSA Free

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Western Blot: MLL5 Antibody [NBP3-03559] - Western blot analysis of extracts of C2C12 cells, using (NBP3-03559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. ...read more
Immunohistochemistry: MLL5 Antibody [NBP3-03559] - Analysis of mouse brain using MLL5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MLL5 Antibody [NBP3-03559] - Paraffin-embedded human breast cancer using MLL5 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry-Paraffin: MLL5 Antibody [NBP3-03559] - Paraffin-embedded rat ovary using MLL5 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry-Paraffin: MLL5 Antibody [NBP3-03559] - Paraffin-embedded mouse lung using MLL5 antibody at dilution of 1:200 (40x lens).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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MLL5 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human MLL5 (NP_061152.3). VELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQHISKCKKAFREGSRKSSRVKGSAPEIDPSSDGSNFGWETKIKAWMDRYEEANNNQYSEGVQREAQRIALRLGNGNDKKEMNKSDLNTNNLLFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITIAFDFDYGNCKYKVDCACLKENPECPVLKRSSESMENINSGYETRRKKGKKDKDISK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KMT2E
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for MLL5 Antibody - Azide and BSA Free

  • EC 2.1.1.43
  • FLJ10078
  • HDCMC04P
  • histone-lysine N-methyltransferase MLL5
  • KMT2EFLJ14026
  • Lysine N-methyltransferase 2E
  • MGC70452
  • myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)
  • Myeloid/lymphoid or mixed-lineage leukemia protein 5

Background

MLL5 is a divergent member of the Drosophila Trithorax related SET and PHD domain containing chromatin regulators that are involved in the regulation of transcriptional "memory" during differentiation. Human MLL5 is located on chromosome 7q22, that is frequently deleted in myeloid leukemias. Inactivation of the Mll5 gene in mice results in a reduction in the average representation of hematopoietic stem cells, and in functional impairment of long term hematopoietic repopulation potential under competitive conditions. Overexpression of the protein also inhibits cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional MLL5 Products

Array NBP3-03559

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Bioinformatics

Gene Symbol KMT2E