Mitofusin 2 Antibody (S153-5) Summary
Immunogen |
Fusion protein amino acids 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2. Rat: 97% identity (226/231 amino acids identical). Human: 92% identity (214/231 amino acids identical) ~55% identity with Mitofusin-1. |
Localization |
Mitochondrion , Mitochondrion Outer Membrane |
Specificity |
Approx 55% identity with Mitofusin-1.Detects approx 90kDa. No cross-reactivity against Mitofusin-1. |
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
MFN2 |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:100
- Immunohistochemistry
- Western Blot 1:1000
|
Application Notes |
1 ug/ml of Mitofusin 2 Antibody was sufficient for detection of Mitofusin-2 in 20 ug of mouse cardiac mitochondrial lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
Reactivity Notes
Immunogen displays sequence identity for non-tested species: Mouse and Rat.
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.4), 50% Glycerol |
Preservative |
0.1% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Protein G purified |
Alternate Names for Mitofusin 2 Antibody (S153-5)
Background
MFN2 encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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