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MICL/CLEC12A Antibody [Unconjugated]

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M1 mouse myeloid leukemia cell line was stained with Goat Anti-Mouse MICL/CLEC12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2950, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
M1 mouse myeloid leukemia cell line was stained with Goat Anti-Mouse MICL/CLEC12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2950, filled histogram) or control antibody (Catalog # AB-108-C, open ...read more
MICL/CLEC12A was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse MICL/CLEC12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2950) at 15 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2950
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2950-SP
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MICL/CLEC12A Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MICL/CLEC12A
Thr67-Arg267
Accession # NP_808354
Specificity
Detects recombinant mouse MICL/CLEC12A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
1% cross‑reactivity with recombinant human MICL is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CLEC12A
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2950 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICL/CLEC12A Antibody [Unconjugated]

  • CD371
  • CLEC12A
  • CLL1
  • CLL-1
  • C-type lectin domain family 12, member A
  • DCAL-2
  • MICL

Background

MICL, also known as killer cell lectin-like receptor 1 (KLR1), is an inhibitory C-type lectin-like receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). It is a75 kDa type II membrane protein that is expressed on neutrophils, NK cells, monocytes and dendritic cells. The amino acid sequence of mouse MICL extracellular domain is 53%, 52% and 58% identical to that of human, bovine and canine MICL, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MICL/CLEC12A Antibody (AF2950)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CLEC12A
Uniprot