![Immunohistochemistry: mGluR1 Antibody [NB100-93555] - Immunohistochemical detection of mGluR and 5-HTR subtypes in dLGN of Galphaq/Galpha11-/-. mGluR1alpha-specific antibodies caused strong staining. 5-HT2CR and mGluR1alpha did not appear to be somatically expressed. Scale bars indicate 100 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2010.00132/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mGluR1-Antibody-Immunohistochemistry-NB100-93555-img0006.jpg "Immunohistochemistry: mGluR1 Antibody [NB100-93555] - Immunohistochemical detection of mGluR and 5-HTR subtypes in dLGN of Galphaq/Galpha11-/-. mGluR1alpha-specific antibodies caused strong staining. 5-HT2CR and mGluR1alpha did not appear to be somatically expressed. Scale bars indicate 100 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2010.00132/abstract) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide comprising internal residues of the human metabotropic GluR1 alpha protein. Reacts with rat and mouse GluR1. |
| Specificity | mGluR1, Synthetic peptide comprising internal residues of the human GluR1 alpha protein. Reacts with rat and mouse mGluR1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GRM1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody is useful for Western blot 1:500-1:1000, and immunohistochemistry 1:100-1:500. Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Frozen were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 132.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-93555 | Applications | Species |
|---|---|---|
| Coulon P, Kanyshkova T, Broicher T et al. Activity Modes in Thalamocortical Relay Neurons are Modulated by G(q)/G(11) Family G-proteins - Serotonergic and Glutamatergic Signaling. Front Cell Neurosci 2010-10-25 [PMID: 21267426] (IHC-Fr, ICC/IF, Mouse) | IHC-Fr, ICC/IF | Mouse |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: mGluR1 Antibody [NB100-93555] - Detection of metabotropic Glutamate Receptor 1a protein in 20ug of human brain lysate (lane 2) with the use of NB100-93555 as a primary antibody at 1:1000 dilution and an AP conjugated secondary antibody at 1:5000 dilution. Lane 1 represents the MW marker.](http://images.novusbio.com/fullsize/mGluR1-Antibody-Western-Blot-NB100-93555-img0002.jpg "Western Blot: mGluR1 Antibody [NB100-93555] - Detection of metabotropic Glutamate Receptor 1a protein in 20ug of human brain lysate (lane 2) with the use of NB100-93555 as a primary antibody at 1:1000 dilution and an AP conjugated secondary antibody at 1:5000 dilution. Lane 1 represents the MW marker.")
![Immunohistochemistry: mGluR1 Antibody [NB100-93555] - Recommended dilution for IHC is 1:100-500. However, the investigator should determine the optimal dilution for a specific application.](http://images.novusbio.com/fullsize/mGluR1-Antibody-Immunohistochemistry-NB100-93555-img0001.jpg "Immunohistochemistry: mGluR1 Antibody [NB100-93555] - Recommended dilution for IHC is 1:100-500. However, the investigator should determine the optimal dilution for a specific application.")
![Immunohistochemistry: mGluR1 Antibody [NB100-93555] - Immunohistochemical detection of mGluR and 5-HTR subtypes in dLGN of Galpha11-/- mice. mGluR1alpha-specific antibodies caused strong staining as in the dLGN of Galphaq/Galpha11-/-mice. Scale bars indicate 100 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2010.00132/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mGluR1-Antibody-Immunohistochemistry-NB100-93555-img0004.jpg "Immunohistochemistry: mGluR1 Antibody [NB100-93555] - Immunohistochemical detection of mGluR and 5-HTR subtypes in dLGN of Galpha11-/- mice. mGluR1alpha-specific antibodies caused strong staining as in the dLGN of Galphaq/Galpha11-/-mice. Scale bars indicate 100 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2010.00132/abstract) licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: mGluR1 Antibody - BSA Free [NB100-93555] - Immunohistochemical staining of mGluR1 alpha & MAP2. Neurons of the dLGN were stained using specific antibodies against microtubule associated protein 2 (MAP2) & against mGluR1 alpha conjugated to Cy2 & Cy3, respectively. While MAP2 was clearly present in the soma, mGluR1 alpha appears to be absent there. Scale bars indicate 10 μm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2010.00132/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-93555_rabbit-polyclonal-mglur1-antibody-310202415394537.jpg "Immunocytochemistry/ Immunofluorescence: mGluR1 Antibody - BSA Free [NB100-93555] - Immunohistochemical staining of mGluR1 alpha & MAP2. Neurons of the dLGN were stained using specific antibodies against microtubule associated protein 2 (MAP2) & against mGluR1 alpha conjugated to Cy2 & Cy3, respectively. While MAP2 was clearly present in the soma, mGluR1 alpha appears to be absent there. Scale bars indicate 10 μm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2010.00132/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")